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MMP-13 (MM0019-12E10)

SC-101564
Santa Cruz Biotechnology
ApplicationsImmunoFluorescence, Western Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityHuman
TargetMMP13
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100 ug
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Overview

  • Supplier
    Santa Cruz Biotechnology
  • Product Name
    MMP-13 (MM0019-12E10)
  • Delivery Days Customer
    5
  • Applications
    ImmunoFluorescence, Western Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Gene ID4322
  • Target name
    MMP13
  • Target description
    matrix metallopeptidase 13
  • Target synonyms
    CLG3, MANDP1, MDST, MMP-13, collagenase 3, matrix metalloproteinase 13 (collagenase 3)
  • Host
    Mouse
  • Isotype
    IgG1
  • Reactivity
    Human
  • Storage Instruction
    2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of MMP13 using anti-MMP13 antibody (A00420-3). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human A549 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MMP13 antigen affinity purified polyclonal antibody (Catalog # A00420-3) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for MMP13 at approximately 54 kDa. The expected band size for MMP13 is at 54 kDa.
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MMP13 antibody [N3C1], Internal detects MMP13 protein at cytoplasm by immunofluorescent analysis. Sample: NT2D1 cells were fixed in ice-cold MeOH for 5 min. Green: MMP13 protein stained by MMP13 antibody [N3C1], Internal (GTX100665) diluted at 1:500. Blue: Hoechst 33342 staining. Scale bar = 10 μm.
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