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Immunohistochemistry of paraffin-embedded Human cervical cancer tissue using MMP1 Polyclonal Antibody at dilution 1:55
Immunohistochemistry of paraffin-embedded Human cervical cancer tissue using MMP1 Polyclonal Antibody at dilution 1:55
Immunohistochemistry of paraffin-embedded Human cervical cancer tissue using MMP1 Polyclonal Antibody at dilution 1:55

MMP1 Polyclonal Antibody

E-AB-10061
Elabscience
ApplicationsImmunoHistoChemistry
Product group Antibodies
TargetMMP1
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Overview

  • Supplier
    Elabscience
  • Product Name
    MMP1 Polyclonal Antibody
  • Delivery Days Customer
    12
  • Applications
    ImmunoHistoChemistry
  • Applications Supplier
    ELISA IHC
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    0.4 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID4312
  • Target name
    MMP1
  • Target description
    matrix metallopeptidase 1
  • Target synonyms
    CLG, CLGN, interstitial collagenase, fibroblast collagenase, matrix metallopeptidase 1 (interstitial collagenase), matrix metalloprotease 1
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP03956
  • Protein Name
    Interstitial collagenase
  • Scientific Description
    Proteins of the matrix metalloproteinase (MMP) family are involved in the breakdown of extracellular matrix in normal physiological processes, such as embryonic development, reproduction, and tissue remodeling, as well as in disease processes, such as arthritis and metastasis. Most MMPs are secreted as inactive proproteins which are activated when cleaved by extracellular proteinases. This gene encodes a secreted enzyme which breaks down the interstitial collagens, types I, II, and III. The gene is part of a cluster of MMP genes which localize to chromosome 11q22.3. Alternative splicing results in multiple transcript variants.
  • Storage Instruction
    -20°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of MMP1 using anti-MMP1 antibody (A00733-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human placenta tissue lysates, Lane 2: human CACO-2 whole cell lysates, Lane 3: human K562 whole cell lysates, Lane 4: human U20S whole cell lysates, Lane 5: human MCF-7 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MMP1 antigen affinity purified polyclonal antibody (Catalog # A00733-1) at 0.25 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for MMP1 at approximately 54 kDa. The expected band size for MMP1 is at 54 kDa.
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