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Western Blot analysis of Hela cell using MMP13 Polyclonal Antibody at dilution of 1:500
Western Blot analysis of Hela cell using MMP13 Polyclonal Antibody at dilution of 1:500
Western Blot analysis of Hela cell using MMP13 Polyclonal Antibody at dilution of 1:500

MMP13 Polyclonal Antibody

E-AB-10064
Elabscience
ApplicationsWestern Blot
Product group Antibodies
TargetMMP13
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Overview

  • Supplier
    Elabscience
  • Product Name
    MMP13 Polyclonal Antibody
  • Delivery Days Customer
    12
  • Applications
    Western Blot
  • Applications Supplier
    ELISA WB IHC
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    0.4 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID4322
  • Target name
    MMP13
  • Target description
    matrix metallopeptidase 13
  • Target synonyms
    CLG3, MANDP1, MDST, MMP-13, collagenase 3, matrix metalloproteinase 13 (collagenase 3)
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP45452
  • Protein Name
    Collagenase 3
  • Scientific Description
    Proteins of the matrix metalloproteinase (MMP) family are involved in the breakdown of extracellular matrix in normal physiological processes, such as embryonic development, reproduction, and tissue remodeling, as well as in disease processes, such as arthritis and metastasis. Most MMPs are secreted as inactive proproteins which are activated when cleaved by extracellular proteinases. The protein encoded by this gene cleaves type II collagen more efficiently than types I and III. It may be involved in articular cartilage turnover and cartilage pathophysiology associated with osteoarthritis. The gene is part of a cluster of MMP genes which localize to chromosome 11q22.3.
  • Storage Instruction
    -20°C
  • UNSPSC
    41116161

MSDS

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Figure 1. Western blot analysis of MMP13 using anti-MMP13 antibody (A00420-3). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human A549 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MMP13 antigen affinity purified polyclonal antibody (Catalog # A00420-3) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for MMP13 at approximately 54 kDa. The expected band size for MMP13 is at 54 kDa.
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