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ProductsBack/MMP15 antibody [5D11]
IHC-P analysis of formalin fixed human placenta tissue using GTX52481 MMP15 antibody [5D11].
IHC-P analysis of formalin fixed human placenta tissue using GTX52481 MMP15 antibody [5D11].
IHC-P analysis of formalin fixed human placenta tissue using GTX52481 MMP15 antibody [5D11].

MMP15 antibody [5D11]

GTX52481
GeneTex
ApplicationsWestern Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
DownloadDatasheet
Product group Antibodies
TargetMMP15
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Overview

  • Supplier
    GeneTex
  • Product Name
    MMP15 antibody [5D11]
  • Delivery Days Customer
    9
  • Application Supplier Note
    WB: 1:400 - 1:1000. IHC-P: 1:50 - 1:200. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    Western Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    5D11
  • Concentration
    200 ug/ml
  • Conjugate
    Unconjugated
  • Gene ID4324
  • Target name
    MMP15
  • Target description
    matrix metallopeptidase 15
  • Target synonyms
    matrix metallopeptidase 15 (membrane-inserted); matrix metalloproteinase 15 (membrane-inserted); matrix metalloproteinase-15; membrane-type matrix metalloproteinase 2; membrane-type-2 matrix metalloproteinase; MMP-15; MT2MMP; MT2-MMP; MT-MMP 2; MTMMP2; SMCP-2
  • Host
    Mouse
  • Isotype
    IgG1
  • Protein IDP51511
  • Protein Name
    Matrix metalloproteinase-15
  • Scientific Description
    This gene encodes a member of the peptidase M10 family and membrane-type subfamily of matrix metalloproteinases (MMPs). Proteins in this family are involved in the breakdown of extracellular matrix in normal physiological processes, such as embryonic development, reproduction, and tissue remodeling, as well as in disease processes, such as arthritis and metastasis. Members of this subfamily contain a transmembrane domain suggesting that these proteins are expressed at the cell surface rather than secreted. The encoded preproprotein is proteolytically processed to generate the mature protease. This protein may play a role in cancer progression. [provided by RefSeq, Jan 2016]
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of MT2-MMP/MMP15 using anti-MT2-MMP/MMP15 antibody (A06396-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human A431 whole cell lysates, Lane 2: human HEK293 whole cell lysates, Lane 3: human U20S whole cell lysates, Lane 4: rat RH35 whole cell lysates, Lane 5: mouse liver tissue lysates, Lane 6: mouse testis tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MT2-MMP/MMP15 antigen affinity purified polyclonal antibody (Catalog # A06396-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for MT2-MMP/MMP15 at approximately 74KD. The expected band size for MT2-MMP/MMP15 is at 74KD.
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