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The image on the left is immunohistochemistry of paraffin-embedded Human ovarian cancer tissue using CSB-PA104319(MMP20 Antibody) at dilution 1/30, on the right is treated with synthetic peptide. (Original magnification: x200)
The image on the left is immunohistochemistry of paraffin-embedded Human ovarian cancer tissue using CSB-PA104319(MMP20 Antibody) at dilution 1/30, on the right is treated with synthetic peptide. (Original magnification: x200)
The image on the left is immunohistochemistry of paraffin-embedded Human ovarian cancer tissue using CSB-PA104319(MMP20 Antibody) at dilution 1/30, on the right is treated with synthetic peptide. (Original magnification: x200)

MMP20 Antibody

CSB-PA104319
Cusabio
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
Product group Antibodies
TargetMMP20
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Overview

  • Supplier
    Cusabio
  • Product Name
    MMP20 Antibody
  • Delivery Days Customer
    20
  • Applications
    Western Blot, ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID9313
  • Target name
    MMP20
  • Target description
    matrix metallopeptidase 20
  • Target synonyms
    AI2A2; enamel metalloproteinase; matrix metalloproteinase 20 (enamelysin); matrix metalloproteinase-20; MMP-20
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDO60882
  • Protein Name
    Matrix metalloproteinase-20
  • Scientific Description
    Proteins of the matrix metalloproteinase (MMP) family are involved in the breakdown of extracellular matrix in normal physiological processes, such as embryonic development, reproduction, and tissue remodeling, as well as in disease processes, such as arthritis and metastasis. Most MMPs are secreted as inactive proproteins which are activated when cleaved by extracellular proteinases. The protein encoded by this gene degrades amelogenin, the major protein component of dental enamel matrix, and thus thought to play a role in tooth enamel formation. A mutation in this gene, which alters the normal splice pattern and results in premature termination of the encoded protein, has been associated with amelogenesis imperfecta. This gene is part of a cluster of MMP genes located on chromosome 11q22.3.
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of MMP20 using anti-MMP20 antibody (A06145-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human Caco-2 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MMP20 antigen affinity purified polyclonal antibody (Catalog # A06145-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for MMP20 at approximately 54 kDa. The expected band size for MMP20 is at 54 kDa.
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