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Formalin-fixed and paraffin embedded mouse embryo labeled with Rabbit Anti MMP20 Polyclonal Antibody, Unconjugated (bs-5788R) at 1:200 followed by conjugation to the secondary antibody and DAB staining
Formalin-fixed and paraffin embedded mouse embryo labeled with Rabbit Anti MMP20 Polyclonal Antibody, Unconjugated (bs-5788R) at 1:200 followed by conjugation to the secondary antibody and DAB staining
Formalin-fixed and paraffin embedded mouse embryo labeled with Rabbit Anti MMP20 Polyclonal Antibody, Unconjugated (bs-5788R) at 1:200 followed by conjugation to the secondary antibody and DAB staining

MMP20 Polyclonal Antibody

BS-5788R
Bioss
ApplicationsImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
Product group Antibodies
TargetMMP20
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Overview

  • Supplier
    Bioss
  • Product Name
    MMP20 Polyclonal Antibody
  • Delivery Days Customer
    16
  • Applications
    ImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
  • Applications Supplier
    WB(1:300-5000), ELISA(1:500-1000), IHC-P(1:200-400), IHC-F(1:100-500), IF(IHC-P)(1:50-200), IF(IHC-F)(1:50-200), IF(ICC)(1:50-200)
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    1 ug/ul
  • Conjugate
    Unconjugated
  • Gene ID9313
  • Target name
    MMP20
  • Target description
    matrix metallopeptidase 20
  • Target synonyms
    AI2A2, MMP-20, matrix metalloproteinase-20, enamel metalloproteinase, matrix metalloproteinase 20 (enamelysin)
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDO60882
  • Protein Name
    Matrix metalloproteinase-20
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

References

  • Aseervatham J, Geetu S, Anunobi CC, et al. Survey of dentin sialophosphoprotein and its cognate matrix metalloproteinase-20 in human cancers. Cancer Med. 2019,8(5):2167-2178. doi: 10.1002/cam4.2117
    Read this paper
  • Ogbureke KU, Koli K, Saxena G. Matrix Metalloproteinase 20 Co-expression With Dentin Sialophosphoprotein in Human and Monkey Kidneys. J Histochem Cytochem. 2016,64(10):623-36. doi: 10.1369/0022155416665098
    Read this paper
  • Golubenko EA, Golubenko IuE. [Individual cosmetic single-wall prosthesis for the eye]. Oftalmol Zh. 1969,24(3):208-10.
    Read this paper

Datasheet

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The image on the left is immunohistochemistry of paraffin-embedded Human ovarian cancer tissue using CSB-PA104319(MMP20 Antibody) at dilution 1/30, on the right is treated with synthetic peptide. (Original magnification: x200)
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Figure 1. Western blot analysis of MMP20 using anti-MMP20 antibody (A06145-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human Caco-2 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MMP20 antigen affinity purified polyclonal antibody (Catalog # A06145-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for MMP20 at approximately 54 kDa. The expected band size for MMP20 is at 54 kDa.
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