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Western Blot analysis of A549 cells using MNT Polyclonal Antibody
Western Blot analysis of A549 cells using MNT Polyclonal Antibody
Western Blot analysis of A549 cells using MNT Polyclonal Antibody

MNT Antibody

CSB-PA003261
Cusabio
ApplicationsWestern Blot, ELISA
Product group Antibodies
ReactivityHuman
TargetMNT
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Overview

  • Supplier
    Cusabio
  • Product Name
    MNT Antibody
  • Delivery Days Customer
    20
  • Applications
    Western Blot, ELISA
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID4335
  • Target name
    MNT
  • Target description
    MAX network transcriptional repressor
  • Target synonyms
    MAD6, MXD6, ROX, bHLHd3, lncRNA-HAL, max-binding protein MNT, MAX binding protein, MNT, MAX dimerization protein, Max-interacting protein, class D basic helix-loop-helix protein 3, myc antagonist MNT
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ99583
  • Protein Name
    Max-binding protein MNT
  • Reactivity
    Human
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of MNT using anti-MNT antibody (A03357-3). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human Raji whole cell lysates, Lane 3: human A549 whole cell lysates, Lane 4: human HEK293 whole cell lysates, Lane 5: human K562 whole cell lysates, Lane 6: human PC-3 whole cell lysates, Lane 7: human Caco-2 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MNT antigen affinity purified polyclonal antibody (Catalog # A03357-3) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for MNT at approximately 62KD. The expected band size for MNT is at 62KD.
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