![A431 whole cell and nuclear extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with MNT antibody [HL2309] (GTX638472) diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. A431 whole cell and nuclear extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with MNT antibody [HL2309] (GTX638472) diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.](https://www.genetex.com/upload/website/prouct_img/normal/GTX638472/GTX638472_T-44998_20230421_WB_Fraction_23042500_189.webp)
A431 whole cell and nuclear extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with MNT antibody [HL2309] (GTX638472) diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
MNT antibody [HL2309]
GTX638472
ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityHuman, Mouse
TargetMNT
Overview
- SupplierGeneTex
- Product NameMNT antibody [HL2309]
- Delivery Days Customer9
- Application Supplier NoteWB: 1:500-1:3000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
- ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
- CertificationResearch Use Only
- ClonalityMonoclonal
- Clone IDHL2309
- Concentration2 mg/ml
- ConjugateUnconjugated
- Gene ID4335
- Target nameMNT
- Target descriptionMAX network transcriptional repressor
- Target synonymsMAD6, MXD6, ROX, bHLHd3, lncRNA-HAL, max-binding protein MNT, MAX binding protein, MNT, MAX dimerization protein, Max-interacting protein, class D basic helix-loop-helix protein 3, myc antagonist MNT
- HostRabbit
- IsotypeIgG
- Protein IDQ99583
- Protein NameMax-binding protein MNT
- Scientific DescriptionThe Myc/Max/Mad network comprises a group of transcription factors that co-interact to regulate gene-specific transcriptional activation or repression. This gene encodes a protein member of the Myc/Max/Mad network. This protein has a basic-Helix-Loop-Helix-zipper domain (bHLHzip) with which it binds the canonical DNA sequence CANNTG, known as the E box, following heterodimerization with Max proteins. This protein is likely a transcriptional repressor and an antagonist of Myc-dependent transcriptional activation and cell growth. This protein represses transcription by binding to DNA binding proteins at its N-terminal Sin3-interaction domain. [provided by RefSeq, Jul 2008]
- ReactivityHuman, Mouse
- Storage Instruction-20°C or -80°C,2°C to 8°C
- UNSPSC12352203
![Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with MNT antibody [HL2309] (GTX638472) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced. Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with MNT antibody [HL2309] (GTX638472) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.](https://www.genetex.com/upload/website/prouct_img/normal/GTX638472/GTX638472_45061_20230602_WB_23060622_710.webp)
![MNT antibody [HL2309] detects MNT protein at cytoplasm and nucleus by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: MNT stained by MNT antibody [HL2309] (GTX638472) diluted at 1:500. Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] (GTX628802) diluted at 1:1000. MNT antibody [HL2309] detects MNT protein at cytoplasm and nucleus by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: MNT stained by MNT antibody [HL2309] (GTX638472) diluted at 1:500. Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] (GTX628802) diluted at 1:1000.](https://www.genetex.com/upload/website/prouct_img/normal/GTX638472/GTX638472_T-44998_20230512_ICC_IF_23060622_453.webp)
![MNT antibody [HL2309] detects MNT protein at cell membrane and cytoplasm by immunohistochemical analysis. Sample: Paraffin-embedded mouse testis. MNT stained by MNT antibody [HL2309] (GTX638472) diluted at 1:200. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min MNT antibody [HL2309] detects MNT protein at cell membrane and cytoplasm by immunohistochemical analysis. Sample: Paraffin-embedded mouse testis. MNT stained by MNT antibody [HL2309] (GTX638472) diluted at 1:200. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](https://www.genetex.com/upload/website/prouct_img/normal/GTX638472/GTX638472_T-44998_20230829_IHC-P_M_23091319_577.webp)





