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Western Blot Positive WB detected in: Hela whole cell lysate All lanes: MOBP antibody at 4microg/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 21, 10, 24 kDa Observed band size: 21 kDa
Western Blot Positive WB detected in: Hela whole cell lysate All lanes: MOBP antibody at 4microg/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 21, 10, 24 kDa Observed band size: 21 kDa
Western Blot Positive WB detected in: Hela whole cell lysate All lanes: MOBP antibody at 4microg/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 21, 10, 24 kDa Observed band size: 21 kDa

MOBP Antibody

CSB-PA014701LA01HU
Cusabio
ApplicationsImmunoFluorescence, Western Blot, ELISA
Product group Antibodies
TargetMOBP
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Overview

  • Supplier
    Cusabio
  • Product Name
    MOBP Antibody
  • Delivery Days Customer
    20
  • Applications
    ImmunoFluorescence, Western Blot, ELISA
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID4336
  • Target name
    MOBP
  • Target description
    myelin associated oligodendrocyte basic protein
  • Target synonyms
    myelin-associated oligodendrocyte basic protein
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ13875
  • Protein Name
    Myelin-associated oligodendrocyte basic protein
  • Scientific Description
    May play a role in compacting or stabilizing the myelin sheath, possibly by binding the negatively charged acidic phospholipids of the cytoplasmic membrane.
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of MOBP using anti-MOBP antibody (A09516-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HepG2 whole cell lysates, Lane 2: human Caco-2 whole cell lysates, Lane 3: human K562 whole cell lysates, Lane 4: human SH-SY5Y whole cell lysates, Lane 5: rat brain tissue lysates, Lane 6: rat C6 whole cell lysates, Lane 7: mouse brain tissue lysates, Lane 8: mouse Neuro-2a whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MOBP antigen affinity purified polyclonal antibody (Catalog # A09516-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for MOBP at approximately 21 kDa. The expected band size for MOBP is at 21 kDa.
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