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MOG Antibody (YA3277)

HY-P83532
MedChem Express
TargetMOG
Product group Antibodies
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Overview

  • Supplier
    MedChem Express
  • Product Name
    MOG Antibody (YA3277)
  • Delivery Days Customer
    5
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Gene ID4340
  • Target name
    MOG
  • Target description
    myelin oligodendrocyte glycoprotein
  • Target synonyms
    BTN6; BTNL11; MOG alpha-5; MOG AluA; MOG AluB; MOG Ig-AluB; MOGIG2; myelin-oligodendrocyte glycoprotein; NRCLP7
  • Host
    Rabbit
  • Isotype
    IgG
  • Scientific Description
    MOG Antibody (YA3277) is a rabbit-derived non-conjugated IgG antibody (Clone NO.: YA3277), targeting MOG, with a predicted molecular weight of 28 kDa (observed band size: 28 kDa). MOG Antibody (YA3277) can be used for WB, ICC/IF experiment in rat background.
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of Myelin oligodendrocyte glycoprotein using anti-Myelin oligodendrocyte glycoprotein antibody (A03294). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: rat brain tissue lysates, Lane 2: mouse brain tissue lysates, After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Myelin oligodendrocyte glycoprotein antigen affinity purified polyclonal antibody (Catalog # A03294) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Myelin oligodendrocyte glycoprotein at approximately 26KD. The expected band size for Myelin oligodendrocyte glycoprotein is at 28KD.
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