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Monoamine Oxidase B Antibody (YA2039, Rabbit)

HY-P82294
MedChem Express
TargetMAOB
Product group Antibodies
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Overview

  • Supplier
    MedChem Express
  • Product Name
    Monoamine Oxidase B Antibody (YA2039, Rabbit)
  • Delivery Days Customer
    5
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Gene ID4129
  • Target name
    MAOB
  • Target description
    monoamine oxidase B
  • Target synonyms
    amine oxidase [flavin-containing] B, MAO, brain, MAO, platelet, MAO-B, adrenalin oxidase, monoamine oxidase type B, tyramine oxidase
  • Host
    Rabbit
  • Isotype
    IgG
  • Scientific Description
    Monoamine Oxidase B Antibody (YA2039) is a rabbit-derived non-conjugated IgG antibody (Clone NO.: YA2039), targeting Monoamine Oxidase B, with a predicted molecular weight of 59 kDa (observed band size: 59 kDa). Monoamine Oxidase B Antibody (YA2039) can be used for WB, IHC-P experiment in human background.
  • Storage Instruction
    -20°C
  • UNSPSC
    41116161

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Western blot All lanes: MAOB antibody at 9microg/ml Lane 1: Mouse liver tissue Lane 2: HepG2 whole cell lysate Lane 3: A549 whole cell lysate Secondary Goat polyclonal to rabbit IgG at 1/10000 dilution Predicted band size: 59, 47 kDa Observed band size: 59, 27 kDa
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Figure 1. Western blot analysis of MAOB using anti-MAOB antibody (PB9665). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human hepatocellular carcinoma tumor tissue (HCCT) lysates, Lane 2: human hepatocellular carcinoma paracancerous tissue (HCCP) lysates, Lane 3: rat liver tissue lysates, Lane 4: mouse liver tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MAOB antigen affinity purified polyclonal antibody (Catalog # PB9665) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for MAOB at approximately 59 kDa. The expected band size for MAOB is at 59 kDa.
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