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ProductsBack/Mouse anti-Cytokeratin 19 Monoclonal Antibody [BA17]

Mouse anti-Cytokeratin 19 Monoclonal Antibody [BA17]

A500-036A
Bethyl Laboratories
ApplicationsWestern Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
Product group Antibodies
TargetKRT19
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Overview

  • Supplier
    Bethyl Laboratories
  • Product Name
    Mouse anti-Cytokeratin 19 Monoclonal Antibody [BA17]
  • Delivery Days Customer
    9
  • Applications
    Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    BA17
  • Concentration
    100 ug/ml
  • Conjugate
    Unconjugated
  • Estimated Purity
    ...
  • Gene ID3880
  • Target name
    KRT19
  • Target description
    keratin 19
  • Target synonyms
    CK19, K19, K1CS, keratin, type I cytoskeletal 19, 40-kDa keratin intermediate filament, CK-19, cytokeratin 19, keratin 19, type I, keratin, type I, 40-kd
  • Host
    Mouse
  • Isotype
    IgG
  • Protein IDP08727
  • Protein Name
    Keratin, type I cytoskeletal 19
  • Storage Instruction
    2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of Cytokeratin 19 using anti-Cytokeratin 19 antibody (PB9715). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human placenta tissue lysates. Lane 2: human MCF-7 whole cell lysates, Lane 3: human SW620 whole cell lysates, Lane 4: human HepG2 whole cell lysates, Lane 5: human PANC-1 whole cell lysates, Lane 6: rat lung tissue lysates, Lane 7: rat small intestine tissue lysates, Lane 8: mouse lung tissue lysates, Lane 9: mouse HEPA1-6 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Cytokeratin 19 antigen affinity purified polyclonal antibody (Catalog # PB9715) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Cytokeratin 19 at approximately 44 kDa. The expected band size for Cytokeratin 19 is at 44 kDa.
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