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Mouse anti Human CD2, conjugated with Biotin

Research Use Only
0023
Nordic-MUbio
ApplicationsFlow Cytometry
Product group Antibodies
ReactivityHuman
TargetCD2
Price on request
100 tests
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Overview

  • Supplier
    Nordic-MUbio
  • Product Name
    Mouse anti Human CD2, conjugated with Biotin
  • Delivery Days Customer
    7
  • Application Supplier Note
    PBMC: Add10 microl of MAB/10 PBMC in 100 microl PBS. Mix gently and incubate for 15 minutes at 2 to 8oC. Wash twice with PBS and analyze or fix with 0.5% v/v of paraformaldehyde in PBS and analyze. WHOLE BLOOD: Add10 microl of MAB/100 microl of whole blood. Mix gently and incubate for 15 minutes at room temperature 20oC. Lyse the whole blood. Wash once with PBS and analyze or fix with 0.5% v/v of paraformaldehyde in PBS and analyze. . See instrument manufacturers instructions for Lysed Whole Blood and Immunofluorescence analysis with a flow cytometer or microscope. ALLOPHYCOCYANIN: (APC) conjugates are analyzed in multi-color flow cytometry with instruments equipped with a second laser and proper filters. Laser excitation is at 633 nm with a Helium Neon (HeNe) laser or a 600-640 nm (633 nm) range for a Dye laser. Peak fluorescence emission is at 660 nm.
  • Applications
    Flow Cytometry
  • Applications Supplier
    Flow Cytometry
  • Category Supplier
    Primary antibodies
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    T6.3
  • Conjugate
    Biotin
  • Gene ID914
  • Target name
    CD2
  • Target description
    CD2 molecule
  • Target synonyms
    CD2 antigen (p50), sheep red blood cell receptor; erythrocyte receptor; LFA-2; LFA-3 receptor; lymphocyte-function antigen-2; rosette receptor; SRBC; T11; T-cell surface antigen CD2; T-cell surface antigen T11/Leu-5
  • Host
    Mouse
  • Isotype
    IgG2a
  • Protein IDP06729
  • Protein Name
    T-cell surface antigen CD2
  • Scientific Description
    T-cell surface antigen CD2; CD2 cytoplasmic domain-binding protein
  • Shelf life instruction
    See expiration date on vial
  • Reactivity
    Human
  • Reactivity Supplier
    Human
  • Reactivity Supplier Note
    Derived from hybridization of mouse Sp2/0 myeloma cells with spleen cells from BALB/c mice immunized with T Lymphocytes activated by mixed lymphocyte culture.
  • Storage Instruction
    2°C to 8°C
  • UNSPSC
    12352203

References

  • 1. An Improved Rosetting Assay for Detection of Human T Lymphocytes. Kaplan M.E., Clark C., J. Immunol. Methods 1974, 5,131. 2. Structural and functional characterization of the CD2 immunoadhesion domain. Evidence for inclusion of CD2 in an alpha-beta protein folding class.Recny M.A., Neidhardt E.A., Sayre P.H., Ciardelli T.L., Reinherz E.L., J. Biol. Chem. 1990 May 2;265(15):8541-9. 3. Partial deletions of the cytoplasm domain of CD2 result in a partial defect in signal transduction. Bierer B.E., Bogart R.E., Burakoff S.J., J. Immunol. 1990 Feb. :144(3):785. 4. Functional CD2 mutants unable to bind to, or be stimulated by, LFA-3. Wolff H.L., Burakoff S.J., Bierer B.E., J. Immunol. 1990 Feb. 1;144(4):1215-20. 5. Association of CD2 and CD45 on human T lymphocytes. Schraven B., Samstag Y., Altevogt P., Meuer S.C., Nature 1990 May ;345(6270):71-4 . Product Specific References: 1. Gardner, J.P. et al. 'Robust, but transient expression of adeno-associated virus-transduced genes during human t lymphopoisis.' Blood, 1997, 90, 4854-4864.