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ProductsBack/Mouse anti Human CD2 FITC - CD20 PE

Mouse anti Human CD2 FITC - CD20 PE

Research Use Only
0220S
Nordic-MUbio
ApplicationsFlow Cytometry
Product group Antibodies
ReactivityHuman
TargetCD2
Price on request
50 tests
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ISO certified
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Overview

  • Supplier
    Nordic-MUbio
  • Product Name
    Mouse anti Human CD2 FITC - CD20 PE
  • Delivery Days Customer
    7
  • Application Supplier Note
    PBMC: Add10 microl of MAB/10^6 PBMC in 100 microl PBS. Mix gently and incubate for 15 minutes at 2 to 8 C. Wash twice with PBS and analyze or fix with 0.5% v/v of paraformaldehyde in PBS and analyze. WHOLE BLOOD: Add10 microl of MAB/100 microl of whole blood. Mix gently and incubate for 15 minutes at room temperature 20 C. Lyse the whole blood. Wash once with PBS and analyze or fix with 0.5% v/v of paraformaldehyde in PBS and analyze. See instrument manufacturers instructions for Lysed Whole Blood and Immunofluorescence analysis with a flow cytometer or microscope.
  • Applications
    Flow Cytometry
  • Applications Supplier
    Flow Cytometry
  • Category Supplier
    Primary antibodies
  • Certification
    Research Use Only
  • Conjugate
    FITC, RPE
  • Gene ID914
  • Target name
    CD2
  • Target description
    CD2 molecule
  • Target synonyms
    CD2 antigen (p50), sheep red blood cell receptor; erythrocyte receptor; LFA-2; LFA-3 receptor; lymphocyte-function antigen-2; rosette receptor; SRBC; T11; T-cell surface antigen CD2; T-cell surface antigen T11/Leu-5
  • Host
    Mouse
  • Isotype
    IgG1, IgG2a
  • Protein IDP06729
  • Protein Name
    T-cell surface antigen CD2
  • Scientific Description
    CD2 FITC - CD20 PE
  • Shelf life instruction
    See expiration date on vial
  • Reactivity
    Human
  • Reactivity Supplier
    Human
  • Reactivity Supplier Note
    CD2=Derived from the hybridization of mouse Sp2/0 myeloma cells with spleen cells from BALB/c mice immunized with t lymphocytes activated by mixed lymphocyte culture.CD20=Derived from the hybridization of mouse Sp2/0 myeloma cells with spleen cells from BALB/c mice immunized with the LB lymphoblastoid cell line.
  • Storage Instruction
    2°C to 8°C
  • UNSPSC
    12352203

References

  • 1.An Improved Rosetting Assay for Detection of Human T Lymphocytes. Kaplan M.E., Clark C., J. Immunol. Methods 1974, 5,131 2.Structural and functional characterization of the CD2 immunoadhesion domain. Evidence for inclusion of CD2 in an alpha-beta protein folding class. Recny M.A., Neidhardt E.A., Sayre P.H., Ciardelli T.L., Reinherz E.L., J. Biol. Chem. 1990 May 2;265(15):85419 3. Partial deletions of the cytoplasm domain of CD2 result in a partial defect in signal transduction. Bierer B.E., Bogart R.E., Burakoff S.J., J. Immunol. 1990 Feb. :144(3):785 4. Functional CD2 mutants unable to bind to, or be stimulated by, LFA-3. Wolff H.L., Burakoff S.J., Bierer B.E., J. Immunol. 1990 Feb. 1;144(4):1215-20 5. Association of CD2 and CD45 on human T lymphocytes. Schraven B., Samstag Y., Altevogt P., Meuer S.C., Nature 1990 May ;345(6270):71-4 6. In vivo and in vitro expression of myeloid antigens on B-lineage acute lymphoblastic leukemia cells. Leukemia 1991 Ja;5(1):19-25 Hara J; Kawa-Ha K; Yumura-Yagi K; Kurahashi H; Tawa A; Ishihara S; Inoue M; Murayama N; Okada S. 7. Activation of dense human tonsilar B cells. Induction of c-myc gene expression via two distinct signal transduction pathways. J Immunol 1991 Feb ;146(3):846-53 White MW; McConnell F; Shu GL; Morris DR; Clark EA. 8. Differential effects of low and high concentrations of interleukin 6 on human B cells. Eur J Immunol 1990 No;20(11):2389-93 Levy Y; Fermand JP; Brouet JC. 9. Immunophenotypes in 'classical' B-cell chronic lymphocytic leukemia. Correlation with normal cellular counterpart and clinical findings. Cancer 1990 Oct 1;66(8):1738-42 Baldini L; Cro L; Cortelezzi A; Calori R; Nobili L; Maiolo AT; Polli EE. 10. B-cell differentiation following autologous, conventional, or T-cell depleted bone marrow transplantation: a recapitulation of normal B-cell ontogeny. Blood 1990 Oct 1;76(8):1647-56 Small TN; Keever CA; Weiner-Fedus S; Heller G; O'Reilly RJ; Flomenberg N. 11. Phenotypic analysis of a large number of normal human bone marrow sample by flow cytometry. Blut 1990 No;61(5):271-7 Andreoni C; Rigal D; Bonnard M; Bernaud J. 12. Identification and characterization of plasma cells in normal human bone marrow by high-resolution flow cytometry. Blood 1990 Nov ;76(9):1739-47 Terstappen LW; Johnsen S; Segers-Nolten IM; Loken MR.