Mouse anti Human CD35, conjugated with FITC

Nordic-MUbio

Mouse anti Human CD35, conjugated with FITC

7

PBMC: Add10 microl of MAB/10^6 PBMC in 100 microl PBS. Mix gently and incubate for 15 minutes at 2 to 8C. Wash twice with PBS and analyze or fix with 0.5% v/v of paraformaldehyde in PBS and analyze. WHOLE BLOOD: Add 10 microl of MAB/100 microl of whole blood. Mix gently and incubate for 15 minutes at room temperature (20C). Lyse the whole blood. Wash once with PBS and analyze or fix with 0.5% v/v of paraformaldehyde in PBS and analyze. See instrument manufacturer?s instructions for Lysed Whole Blood and Immunofluorescence analysis with a flow cytometer or microscope.

Flow Cytometry

Flow Cytometry

Primary antibodies

Research Use Only

Monoclonal

AR2

FITC

CR1

complement C3b/C4b receptor 1 (Knops blood group)

C3b/C4b receptor; C3-binding protein; C3BR; C4BR; CD35; CD35 antigen; complement component (3b/4b) receptor 1 (Knops blood group); complement receptor 1; complement receptor type 1; KN; Knops blood group antigen

Mouse

IgG1

Complement receptor type 1

CD35 FITC

See expiration date on vial

Human

Human

CD35=Derived from the hybridization od mouse SP2/0 cells with spleen cells of mice immunized with purified CR1

2°C to 8°C

12352203