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ProductsBack/Mouse anti Human CD35, conjugated with FITC

Mouse anti Human CD35, conjugated with FITC

Research Use Only
0352
Nordic-MUbio
ApplicationsFlow Cytometry
Product group Antibodies
ReactivityHuman
TargetCR1
Price on request
100 tests
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More than 130 suppliers
ISO certified
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Overview

  • Supplier
    Nordic-MUbio
  • Product Name
    Mouse anti Human CD35, conjugated with FITC
  • Delivery Days Customer
    7
  • Application Supplier Note
    PBMC: Add10 microl of MAB/10^6 PBMC in 100 microl PBS. Mix gently and incubate for 15 minutes at 2 to 8C. Wash twice with PBS and analyze or fix with 0.5% v/v of paraformaldehyde in PBS and analyze. WHOLE BLOOD: Add 10 microl of MAB/100 microl of whole blood. Mix gently and incubate for 15 minutes at room temperature (20C). Lyse the whole blood. Wash once with PBS and analyze or fix with 0.5% v/v of paraformaldehyde in PBS and analyze. See instrument manufacturer?s instructions for Lysed Whole Blood and Immunofluorescence analysis with a flow cytometer or microscope.
  • Applications
    Flow Cytometry
  • Applications Supplier
    Flow Cytometry
  • Category Supplier
    Primary antibodies
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    AR2
  • Conjugate
    FITC
  • Gene ID1378
  • Target name
    CR1
  • Target description
    complement C3b/C4b receptor 1 (Knops blood group)
  • Target synonyms
    C3b/C4b receptor; C3-binding protein; C3BR; C4BR; CD35; CD35 antigen; complement component (3b/4b) receptor 1 (Knops blood group); complement receptor 1; complement receptor type 1; KN; Knops blood group antigen
  • Host
    Mouse
  • Isotype
    IgG1
  • Protein IDP17927
  • Protein Name
    Complement receptor type 1
  • Scientific Description
    CD35 FITC
  • Shelf life instruction
    See expiration date on vial
  • Reactivity
    Human
  • Reactivity Supplier
    Human
  • Reactivity Supplier Note
    CD35=Derived from the hybridization od mouse SP2/0 cells with spleen cells of mice immunized with purified CR1
  • Storage Instruction
    2°C to 8°C
  • UNSPSC
    12352203

References

  • 1. Differential effects of the stimulation of complement receptors CR1 (CD35) and CR2 (CD21) on cell proliferation and intracellular Ca2+ mobilization of chronic lymphocytic leukemia B cells. Hivroz C; Fischer E; Kazatchkine MD; Grillot-Courvalin C J Immunol 1991 Mar 1;146(6):1766-72 2. The C3b/C4b receptor is recognized by the Knops, McCoy, Swain-langley, and York blood group antisera. Moulds JM; Nickells MW; Moulds JJ; Brown MC; Atkinson JP J Exp Med 1991 May ;173(5):1159-63 3. Deduction from Wilms' tumour that glomerular podocytes produce the basement membrane material bearing Goodpasture determinants. Droz D; Diebold N; Jan D; Jaubert F J Pathol 1990 De;162(4):323-7 4. Purification and characterization of IgG immunoconglutinins from patients with systemic lupus erythematosus: implications for a regulatory function. Nilsson B; Ekdahl KN; Svarvare M; Bjel Clin Exp Immunol 1990 No;82(2):262-7 5. Granulocyte-macrophage colony-stimulating factor increases synthesis and expression of CR1 and CR3 by human peripheral blood neutrophils. Neuman E; Huleatt JW; Jack RM J Immunol 1990 Nov 1;145(10):3325-32 6. Expression of CR1 (CD35) mRNA in podocytes from adult and fetal human kidneys. Appay MD; Kazatchkine MD; Levi-Strauss M; Hinglais N; Bariety J Kidney Int 1990 Au;38(2):289-93 7. Complement receptor type 1 (C3b/C4b receptor; CD35) and complement receptor type 2 (C3d/Epstein-Barr virus receptor; CD21). Fearon DT; Ahearn JM Curr Top Microbiol Immunol 1990;153 p83-98