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Mouse Anti-Human CD49f-PE

9650-09
SouthernBiotech
ApplicationsFlow Cytometry
Product group Antibodies
ReactivityHuman
TargetITGA6
100 tests
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Overview

  • Supplier
    SouthernBiotech
  • Product Name
    Mouse Anti-Human CD49f-PE
  • Delivery Days Customer
    7
  • Applications
    Flow Cytometry
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    4F10
  • Conjugate
    RPE
  • Gene ID3655
  • Target name
    ITGA6
  • Target description
    integrin subunit alpha 6
  • Target synonyms
    CD49f, ITGA6A, ITGA6B, JEB6, VLA-6, integrin alpha-6, CD49 antigen-like family member F, integrin alpha6B, integrin, alpha 6, very late activation protein 6
  • Host
    Mouse
  • Isotype
    IgG2b
  • Protein IDP23229
  • Protein Name
    Integrin alpha-6
  • Reactivity
    Human
  • Storage Instruction
    2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of ITGA6 using anti-ITGA6 antibody (A01693). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human A431 whole cell lysates, Lane 2: human A549 whole cell lysates, Lane 3: rat kidney tissue lysates, Lane 4: mouse kidney tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ITGA6 antigen affinity purified polyclonal antibody (Catalog # A01693) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ITGA6 at approximately 127 kDa. The expected band size for ITGA6 is at 127 kDa.
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Lane 1: SW480 cell lysates; Lane 2: A549 cell lysates; Lane 3: Huvec cell lysates; Lane 4: A431 cell lysates probed with Integrin alpha 6 Polyclonal Antibody, Unconjugated (bs-2641R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
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