Bio-Connect

Mouse anti Human CD8

0081
Nordic-MUbio
ApplicationsFlow Cytometry
Product group Antibodies
ReactivityHuman
TargetCD8A
100 ug
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Overview

  • Supplier
    Nordic-MUbio
  • Product Name
    Mouse anti Human CD8
  • Delivery Days Customer
    7
  • Application Supplier Note
    PBMC: Add10 microl of MAB/10^6 PBMC in 100 microl PBS. Mix gently and incubate for 15 minutes at 2 to 8C. Wash twice with PBS and analyze or fix with 0.5% v/v of paraformaldehyde in PBS and analyze. WHOLE BLOOD: Add10 microl of MAB/100 microl of whole blood. Mix gently and incubate for 15 minutes at room temperature 20C. Lyse the whole blood. Wash once with PBS and analyze or fix with 0.5% v/v of paraformaldehyde in PBS and analyze. See instrument manufacturers instructions for Lysed Whole Blood and Immunofluorescence analysis with a flow cytometer or microscope. ALLOPHYCOCYANIN: (APC) conjugates are analyzed in multi-color flow cytometry with instruments equipped with a second laser and proper filters. Laser excitation is at 633 nm with a Helium Neon (HeNe) laser or a 600-640 nm (633 nm) range for a Dye laser. Peak fluorescence emission is at 660 nm. RPE-Cy-5 +: Excites at 488nm and emits at 670nm. Store protected from light.
  • Applications
    Flow Cytometry
  • Applications Supplier
    Flow Cytometry
  • Category Supplier
    Primary antibodies
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    17D8
  • Conjugate
    Unconjugated
  • Gene ID925
  • Target name
    CD8A
  • Target description
    CD8a molecule
  • Target synonyms
    CD8; CD8 antigen, alpha polypeptide (p32); Leu2; Leu2 T-lymphocyte antigen; OKT8 T-cell antigen; p32; T cell co-receptor; T8 T-cell antigen; T-cell antigen Leu2; T-cell surface glycoprotein CD8 alpha chain; T-lymphocyte differentiation antigen T8/Leu-2
  • Host
    Mouse
  • Isotype
    IgG1
  • Protein IDP01732
  • Protein Name
    T-cell surface glycoprotein CD8 alpha chain
  • Scientific Description
    CD8*
  • Shelf life instruction
    See expiration date on vial
  • Reactivity
    Human
  • Reactivity Supplier
    Human
  • Reactivity Supplier Note
    CD8=Derived from the hybridization of mouse NS-1 myeloma cells with spleen cells from BALB/c mice immunized with human perherial blood T lymphocytes.
  • UNSPSC
    12352203