Mouse anti Human CD8
0081
ApplicationsFlow Cytometry
Product group Antibodies
ReactivityHuman
TargetCD8A
Overview
- SupplierNordic-MUbio
- Product NameMouse anti Human CD8
- Delivery Days Customer7
- Application Supplier NotePBMC: Add10 microl of MAB/10^6 PBMC in 100 microl PBS. Mix gently and incubate for 15 minutes at 2 to 8C. Wash twice with PBS and analyze or fix with 0.5% v/v of paraformaldehyde in PBS and analyze. WHOLE BLOOD: Add10 microl of MAB/100 microl of whole blood. Mix gently and incubate for 15 minutes at room temperature 20C. Lyse the whole blood. Wash once with PBS and analyze or fix with 0.5% v/v of paraformaldehyde in PBS and analyze. See instrument manufacturers instructions for Lysed Whole Blood and Immunofluorescence analysis with a flow cytometer or microscope. ALLOPHYCOCYANIN: (APC) conjugates are analyzed in multi-color flow cytometry with instruments equipped with a second laser and proper filters. Laser excitation is at 633 nm with a Helium Neon (HeNe) laser or a 600-640 nm (633 nm) range for a Dye laser. Peak fluorescence emission is at 660 nm. RPE-Cy-5 +: Excites at 488nm and emits at 670nm. Store protected from light.
- ApplicationsFlow Cytometry
- Applications SupplierFlow Cytometry
- Category SupplierPrimary antibodies
- CertificationResearch Use Only
- ClonalityMonoclonal
- Clone ID17D8
- ConjugateUnconjugated
- Gene ID925
- Target nameCD8A
- Target descriptionCD8a molecule
- Target synonymsCD8; CD8 antigen, alpha polypeptide (p32); Leu2; Leu2 T-lymphocyte antigen; OKT8 T-cell antigen; p32; T cell co-receptor; T8 T-cell antigen; T-cell antigen Leu2; T-cell surface glycoprotein CD8 alpha chain; T-lymphocyte differentiation antigen T8/Leu-2
- HostMouse
- IsotypeIgG1
- Protein IDP01732
- Protein NameT-cell surface glycoprotein CD8 alpha chain
- Scientific DescriptionCD8*
- Shelf life instructionSee expiration date on vial
- ReactivityHuman
- Reactivity SupplierHuman
- Reactivity Supplier NoteCD8=Derived from the hybridization of mouse NS-1 myeloma cells with spleen cells from BALB/c mice immunized with human perherial blood T lymphocytes.
- UNSPSC12352203