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ProductsBack/Mouse anti Human LEF-1 transactivation domain

Mouse anti Human LEF-1 transactivation domain

X1074M
Nordic-MUbio
ApplicationsWestern Blot
Product group Antibodies
ReactivityHuman
TargetLEF1

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Overview

  • Supplier
    Nordic-MUbio
  • Product Name
    Mouse anti Human LEF-1 transactivation domain
  • Delivery Days Customer
    7
  • Application Supplier Note
    Antibody detects amino acids 236-242 of human LEF-1. Epitope is missing in some splice variants. Optimal concentration should be evaluated by serial dilutions.
  • Applications
    Western Blot
  • Applications Supplier
    Western Blotting
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    3A12
  • Conjugate
    Unconjugated
  • Gene ID51176
  • Target name
    LEF1
  • Target description
    lymphoid enhancer binding factor 1
  • Target synonyms
    ECTD1, ECTD17, LEF-1, TCF10, TCF1ALPHA, TCF7L3, lymphoid enhancer-binding factor 1, T cell-specific transcription factor 1-alpha, TCF1-alpha
  • Host
    Mouse
  • Isotype
    IgG1
  • Protein IDQ9UJU2
  • Protein Name
    Lymphoid enhancer-binding factor 1
  • Scientific Description
    Anti-Lymphoid Enhancer Factor-1, Transactivation Domain (amino acids 236-242) Monoclonal Antibody
  • Shelf life instruction
    See expiration date on vial
  • Source
    Hybridoma produced by the fusion of splenocytes from mice immunized with synthetic peptide corresponding to amino acids 236-242 of human LEF-1 and mouse myeloma cells
  • Reactivity
    Human
  • Reactivity Supplier
    Human
  • Reactivity Supplier Note
    .
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of LEF1 using anti-LEF1 antibody (A00605-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Jurkat whole cell lysates, Lane 2: mouse thymus tissue lysates, Lane 3: rat C6 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-LEF1 antigen affinity purified polyclonal antibody (Catalog # A00605-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for LEF1 at approximately 58 kDa. The expected band size for LEF1 is at 58 kDa.
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