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Anti-LONRF1 Antibody

101-10928
RayBiotech
ApplicationsWestern Blot, ELISA
Product group Antibodies
TargetLONRF1
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Overview

  • Supplier
    RayBiotech
  • Product Name
    Anti-LONRF1 Antibody
  • Delivery Days Customer
    4
  • Applications
    Western Blot, ELISA
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Gene ID91694
  • Target name
    LONRF1
  • Target description
    LON peptidase N-terminal domain and ring finger 1
  • Target synonyms
    RNF191, LON peptidase N-terminal domain and RING finger protein 1, RING finger protein 191
  • Host
    Mouse
  • Isotype
    IgA
  • Scientific Description
    Human LONRF1 monoclonal antibody (200 uL)
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of LONRF1 using anti-LONRF1 antibody (A14796). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human RT4 whole cell lysates, Lane 3: human HEL whole cell lysates, Lane 4: human HepG2 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-LONRF1 antigen affinity purified polyclonal antibody (Catalog # A14796) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for LONRF1 at approximately 100 kDa. The expected band size for LONRF1 is at 87 kDa.
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