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ProductsBack/Mouse anti Human p34 cdc-2 nuclear protein

Mouse anti Human p34 cdc-2 nuclear protein

X1236M
Nordic-MUbio
ApplicationsImmunoPrecipitation, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
TargetCDK1
100 ug
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Overview

  • Supplier
    Nordic-MUbio
  • Product Name
    Mouse anti Human p34 cdc-2 nuclear protein
  • Delivery Days Customer
    7
  • Application Supplier Note
    Detects three bands within the 34 kDa region corresponding to p34 and its cleavage products. Can also be used for IHC on paraffin-embedded tissue (10 microg/ml) as well as immunocytochemistry and immunoprecipitation. Optimal concentration should be evaluated by serial dilutions.
  • Applications
    ImmunoPrecipitation, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
  • Applications Supplier
    Immunohistochemistry (paraffin);Immunocytochemistry;Western Blotting;Immunoprecipitation;Immunohistochemistry (paraffin);Western Blotting
  • Category Supplier
    Primary antibodies
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    POH-1
  • Conjugate
    Unconjugated
  • Gene ID983
  • Target name
    CDK1
  • Target description
    cyclin dependent kinase 1
  • Target synonyms
    CDC2; CDC28A; cell cycle controller CDC2; cell division control protein 2 homolog; cell division cycle 2, G1 to S and G2 to M; cell division protein kinase 1; cyclin-dependent kinase 1; p34 protein kinase; P34CDC2
  • Host
    Mouse
  • Isotype
    IgG2a
  • Protein IDP06493
  • Protein Name
    Cyclin-dependent kinase 1
  • Shelf life instruction
    See expiration date on vial
  • Reactivity Supplier
    Human;Bovine;Xenopus;Mouse;Rat
  • Reactivity Supplier Note
    Hybridoma produced by the fusion of splenocytes from BALB/c mice immunized with recombinant human p34.cdc2 fusion protein and mouse myeloma cells.
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of CDK1 using anti-CDK1 antibody (PB9533). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. Lane 1: Rat Thymus Tissue Lysate at 50ug, Lane 2: Rat Spleen Tissue Lysate at 50ug, Lane 3: MCF-7 Whole Cell Lysate at 40ug, Lane 4: HELA Whole Cell Lysate at 40ug, Lane 5: JURKAT Whole Cell Lysate at 40ug, Lane 6: NIH3T3 Whole Cell Lysate at 40ug. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CDK1 antigen affinity purified polyclonal antibody (Catalog # PB9533) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002)?with Tanon 5200 system. A specific band was detected for CDK1 at approximately 34 kDa. The expected band size for CDK1 is at 34 kDa.
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