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Anti-ZW10 Antibody

101-11999
RayBiotech
ApplicationsWestern Blot, ELISA
Product group Antibodies
TargetZW10
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Overview

  • Supplier
    RayBiotech
  • Product Name
    Mouse Anti-Human ZW10
  • Delivery Days Customer
    4
  • Applications
    Western Blot, ELISA
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Gene ID9183
  • Target name
    ZW10
  • Target description
    zw10 kinetochore protein
  • Target synonyms
    HZW10, KNTC1AP, centromere/kinetochore protein zw10 homolog, ZW10 homolog, centromere/kinetochore protein, ZW10, kinetochore associated, homolog, zeste white 10 homolog
  • Host
    Mouse
  • Isotype
    IgG2a
  • Scientific Description
    Human ZW10 monoclonal antibody (100 ug)
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of ZW10 using anti-ZW10 antibody (A04266-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human K562 whole cell lysates, Lane 3: human Jurkat whole cell lysates, Lane 4: human CACO-2 whole cell lysates, Lane 5: human HL-60 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ZW10 antigen affinity purified polyclonal antibody (Catalog # A04266-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ZW10 at approximately 95 kDa. The expected band size for ZW10 is at 89 kDa.
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