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WB analysis of PANC1 cells using GTX44856 ZW10 antibody at 0.2-1microg/ml.
WB analysis of PANC1 cells using GTX44856 ZW10 antibody at 0.2-1microg/ml.
WB analysis of PANC1 cells using GTX44856 ZW10 antibody at 0.2-1microg/ml.

ZW10 antibody, N-term

GTX44856
GeneTex
ApplicationsWestern Blot
Product group Antibodies
TargetZW10
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Overview

  • Supplier
    GeneTex
  • Product Name
    ZW10 antibody, N-term
  • Delivery Days Customer
    9
  • Application Supplier Note
    WB: 0.2-2.5 ug/ml. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    Western Blot
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    0.5-1 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID9183
  • Target name
    ZW10
  • Target description
    zw10 kinetochore protein
  • Target synonyms
    HZW10, KNTC1AP, centromere/kinetochore protein zw10 homolog, ZW10 homolog, centromere/kinetochore protein, ZW10, kinetochore associated, homolog, zeste white 10 homolog
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDO43264
  • Protein Name
    Centromere/kinetochore protein zw10 homolog
  • Scientific Description
    This gene encodes a protein that is one of many involved in mechanisms to ensure proper chromosome segregation during cell division. This protein is an essential component of the mitotic checkpoint, which prevents cells from prematurely exiting mitosis. [provided by RefSeq, Aug 2011]
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of ZW10 using anti-ZW10 antibody (A04266-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human K562 whole cell lysates, Lane 3: human Jurkat whole cell lysates, Lane 4: human CACO-2 whole cell lysates, Lane 5: human HL-60 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ZW10 antigen affinity purified polyclonal antibody (Catalog # A04266-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ZW10 at approximately 95 kDa. The expected band size for ZW10 is at 89 kDa.
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