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Mouse anti Melanoma Associated Antigen

MUB1205P
Nordic-MUbio
ApplicationsImmunoFluorescence, ELISA, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
Product group Antibodies
TargetCD63
0.1 mg
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Overview

  • Supplier
    Nordic-MUbio
  • Product Name
    Mouse anti Melanoma Associated Antigen
  • Delivery Days Customer
    7
  • Application Supplier Note
    NKI-C3 is useful for ELISA, immunofluorescence and immuohistochemistry on frozen and paraffin embedded sections. Optimal antibody dilution should be determined by titration.
  • Applications
    ImmunoFluorescence, ELISA, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
  • Applications Supplier
    ELISA;Immunofluorescence;Immunohistochemistry (frozen);Immunohistochemistry (paraffin)
  • Category Supplier
    Primary antibodies
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    NKI-C3
  • Gene ID967
  • Target name
    CD63
  • Target description
    CD63 molecule
  • Target synonyms
    CD63 antigen; CD63 antigen (melanoma 1 antigen); granulophysin; LAMP-3; lysosomal-associated membrane protein 3; lysosome-associated membrane glycoprotein 3; ME491; melanoma-associated antigen ME491; melanoma-associated antigen MLA1; MLA1; ocular melanoma-associated antigen; OMA81H; tetraspanin-30; TSPAN30; tspan-30
  • Host
    Mouse
  • Isotype
    IgG1
  • Protein IDP08962
  • Protein Name
    CD63 antigen
  • Source
    NKI-C3 is a Mouse monoclonal IgG1 antibody derived by fusion of SP2/0 Mouse myeloma cells with spleen cells from a Balb/c mice, immunized with a predominantly intracellular protein of Human melanoma cells.
  • Reactivity Supplier
    Human
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of CD63 using anti-CD63 antibody (PB9250). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human MDA-MB-453 whole cell lysates, Lane 2: human U87 whole cell lysates, Lane 3: human HL-60 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CD63 antigen affinity purified polyclonal antibody (Catalog # PB9250) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CD63 at approximately 30-60 kDa. The expected band size for CD63 is at 26 kDa.
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