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Anti-Mouse Endostatin Antibody

119-12805
RayBiotech
ApplicationsWestern Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
TargetCOL18A1
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Overview

  • Supplier
    RayBiotech
  • Product Name
    Mouse Anti-Mouse Endostatin
  • Delivery Days Customer
    4
  • Applications
    Western Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Gene ID80781
  • Target name
    COL18A1
  • Target description
    collagen type XVIII alpha 1 chain
  • Target synonyms
    antiangiogenic agent; collagen alpha-1(XVIII) chain; collagen, type XVIII, alpha 1; endostatin; GLCC; KNO; KNO1; KS; multi-functional protein MFP
  • Host
    Mouse
  • Protein IDP39060
  • Protein Name
    Collagen alpha-1(XVIII) chain
  • Scientific Description
    Endostatin Mouse anti-Mouse Monoclonal (Unconjugated) Antibody, (50 ul)
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of COL18A1 using anti-COL18A1 antibody (A01302-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human COLO-320 whole cell lysates, Lane 2: human placenta tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-COL18A1 antigen affinity purified polyclonal antibody (Catalog # A01302-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for COL18A1 at approximately 180KD, 20KD. The expected band size for COL18A1 is at 178KD.
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