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ProductsBack/Mouse anti-p40 Monoclonal Antibody [BC28]

Mouse anti-p40 Monoclonal Antibody [BC28]

A500-028A
Bethyl Laboratories
ApplicationsWestern Blot, ImmunoHistoChemistry
Product group Antibodies
TargetTP63
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Overview

  • Supplier
    Bethyl Laboratories
  • Product Name
    Mouse anti-p40 Monoclonal Antibody [BC28]
  • Delivery Days Customer
    9
  • Applications
    Western Blot, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    BC28
  • Concentration
    250 ug/ml
  • Conjugate
    Unconjugated
  • Estimated Purity
    ...
  • Gene ID8626
  • Target name
    TP63
  • Target description
    tumor protein p63
  • Target synonyms
    AIS, B(p51A), B(p51B), EEC3, KET, LMS, NBP, OFC8, RHS, SHFM4, TP53CP, TP53L, TP73L, p40, p51, p53CP, p63, p73H, p73L, tumor protein 63, amplified in squamous cell carcinoma, chronic ulcerative stomatitis protein, keratinocyte transcription factor KET, transformation-related protein 63, tumor protein p53-competing protein
  • Host
    Mouse
  • Isotype
    IgG
  • Protein IDQ9H3D4
  • Protein Name
    Tumor protein 63
  • Storage Instruction
    2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of p63 using anti-p63 antibody (PB9152). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human A431 whole cell lysates. red to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-p63 antigen affinity purified polyclonal antibody (Catalog # PB9152) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for p63 at approximately 75 kDa. The expected band size for p63 is at 75 kDa.
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