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Western Blot analysis of HeLa K562 MCF7 PC-3 cells using p63 Polyclonal Antibody
Western Blot analysis of HeLa K562 MCF7 PC-3 cells using p63 Polyclonal Antibody
Western Blot analysis of HeLa K562 MCF7 PC-3 cells using p63 Polyclonal Antibody

TP63 Antibody

CSB-PA005081
Cusabio
ApplicationsWestern Blot, ELISA
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetTP63
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Overview

  • Supplier
    Cusabio
  • Product Name
    TP63 Antibody
  • Delivery Days Customer
    20
  • Applications
    Western Blot, ELISA
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID8626
  • Target name
    TP63
  • Target description
    tumor protein p63
  • Target synonyms
    AIS; amplified in squamous cell carcinoma; B(p51A); B(p51B); chronic ulcerative stomatitis protein; EEC3; keratinocyte transcription factor KET; KET; LMS; NBP; OFC8; p40; p51; p53CP; p63; p73H; p73L; RHS; SHFM4; TP53CP; TP53L; TP73L; transformation-related protein 63; tumor protein 63; tumor protein p53-competing protein
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ9H3D4
  • Protein Name
    Tumor protein 63
  • Reactivity
    Human, Mouse, Rat
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

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Immunohistochemical staining of human prostate cancer shows absence of nuclear immunoreactivity in tumor cells, while it is present in the adjacent normal a basal epithelial cells.
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Figure 1. Western blot analysis of p63 using anti-p63 antibody (PB9152). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human A431 whole cell lysates. red to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-p63 antigen affinity purified polyclonal antibody (Catalog # PB9152) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for p63 at approximately 75 kDa. The expected band size for p63 is at 75 kDa.
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