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ProductsBack/Mouse anti Rat SNAP25

Mouse anti Rat SNAP25

X1597M
Nordic-MUbio
ApplicationsWestern Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
TargetSNAP25
100 ug
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Overview

  • Supplier
    Nordic-MUbio
  • Product Name
    Mouse anti Rat SNAP25
  • Delivery Days Customer
    7
  • Application Supplier Note
    Antibody can be used for Western blotting (1-5 microg/ml), immunocytochemistry and immunohistochemistry on paraformaldehyde fixed CNS tissues (1-5 microg/ml). Does not stain reduced proteins. Optimal concentration should be evaluated by serial dilutions.
  • Applications
    Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
  • Applications Supplier
    Western Blotting;Immunohistochemistry (paraffin);Immunocytochemistry
  • Category Supplier
    Primary antibodies
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    S-7B8
  • Conjugate
    Unconjugated
  • Gene ID6616
  • Target name
    SNAP25
  • Target description
    synaptosome associated protein 25
  • Target synonyms
    bA416N4.2; CMS18; dJ1068F16.2; resistance to inhibitors of cholinesterase 4 homolog; RIC4; RIC-4; SEC9; SNAP; SNAP-25; SUP; synaptosomal-associated protein 25; synaptosomal-associated protein, 25kDa; synaptosome associated protein 25kDa
  • Host
    Mouse
  • Isotype
    IgG1
  • Protein IDP60880
  • Protein Name
    Synaptosomal-associated protein 25
  • Scientific Description
    Synaptosome-Associated Protein of 25 kDa
  • Shelf life instruction
    See expiration date on vial
  • Source
    Hybridoma produced by the fusion of splenocytes from BALB/c mice immunized with rat brain synaptosomal membranes and mouse NS1 cells.
  • Reactivity Supplier
    Human;Mouse;Rat;Monkey;Chicken;Cow
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of SNAP25 using anti-SNAP25 antibody (A01625-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human SH-SY5Y whole cell lysates, Lane 2: rat brain tissue lysates, Lane 3: mouse brain tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SNAP25 antigen affinity purified polyclonal antibody (Catalog # A01625-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SNAP25 at approximately 24 kDa. The expected band size for SNAP25 is at 24 kDa.
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