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ProductsBack/Purified Anti-Mouse CD25 Antibody [PC-61.5.3]

Purified Anti-Mouse CD25 Antibody [PC-61.5.3]

AGEL0371
Assay Genie
ApplicationsFlow Cytometry, ImmunoPrecipitation
Product group Antibodies
TargetIl2ra
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Overview

  • Supplier
    Assay Genie
  • Product Name
    Mouse CD25 Monoclonal Antibody [PC-61.5.3] (AGEL0371)
  • Delivery Days Customer
    9
  • Applications
    Flow Cytometry, ImmunoPrecipitation
  • Applications Supplier
    FCM,ICH,IP
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Gene ID16184
  • Target name
    Il2ra
  • Target description
    interleukin 2 receptor, alpha chain
  • Target synonyms
    CD25; I; IL-2 receptor subunit alpha; Il2r; IL-2R alpha chain; IL-2R subunit alpha; IL-2-RA; IL2-RA; interleukin-2 receptor subunit alpha; Ly-4; Ly-43; p55 chain
  • Host
    Rat
  • Protein IDP01590
  • Protein Name
    Interleukin-2 receptor subunit alpha
  • Shelf life instruction
    12 months
  • Source
    Rat
  • Reactivity Supplier
    Mouse
  • Storage Instruction
    2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of IL-2 Receptor alpha/Il2ra using anti-IL-2 Receptor alpha/Il2ra antibody (A00214-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: mouse ANA-1 whole cell lysates, Lane 2: mouse NIH/3T3 whole cell lysates, Lane 3: mouse RAW264.7 whole cell lysates, Lane 4: mouse HEPA1-6 whole cell lysates, Lane 5: rat C6 whole cell lysates, Lane 6: rat NRK whole cell lysates, Lane 7: rat RH35 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-IL-2 Receptor alpha/Il2ra antigen affinity purified polyclonal antibody (Catalog # A00214-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for IL-2 Receptor alpha/Il2ra at approximately 50 kDa. The expected band size for IL-2 Receptor alpha/Il2ra is at 50 kDa.
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