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ProductsBack/Purified Anti-Mouse CD25 Antibody [PC-61.5.3]

Purified Anti-Mouse CD25 Antibody [PC-61.5.3]

AGEL0371
Assay Genie
ApplicationsFlow Cytometry, ImmunoPrecipitation
Product group Antibodies
ReactivityMouse
TargetIl2ra
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Overview

  • Supplier
    Assay Genie
  • Product Name
    Purified Anti-Mouse CD25 Antibody [PC-61.5.3]
  • Delivery Days Customer
    9
  • Applications
    Flow Cytometry, ImmunoPrecipitation
  • Applications Supplier
    FCM,ICH,IP
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    PC-61.5.3
  • Conjugate
    Unconjugated
  • Gene ID16184
  • Target name
    Il2ra
  • Target description
    interleukin 2 receptor, alpha chain
  • Target synonyms
    CD25, Il2r, Ly-43, interleukin-2 receptor subunit alpha, IL-2 receptor subunit alpha, IL-2-RA, IL-2R alpha chain, IL-2R subunit alpha, IL2-RA, p55 chain
  • Host
    Rat
  • Protein IDP01590
  • Protein Name
    Interleukin-2 receptor subunit alpha
  • Scientific Description
    Purified Anti-Mouse CD25 Antibody [PC-61.5.3] is a high-performance monoclonal that specifically targets Mouse CD25, delivering exceptional specificity and reliability for your research applications. Rigorously validated for FCM, ICH, IP, this antibody ensures consistent, reproducible results across multiple experimental platforms. Demonstrates excellent reactivity with Mouse samples, providing researchers with confidence in cross-species compatibility. Conveniently packaged in 100microg format to meet your experimental needs. For optimal performance, store at 4°C and maintains stability for 12 months. Backed by rigorous quality control testing to ensure superior performance in your critical research applications.
  • Shelf life instruction
    12 months
  • Source
    Rat
  • Reactivity
    Mouse
  • Reactivity Supplier
    Mouse
  • Storage Instruction
    2°C to 8°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of IL-2 Receptor alpha/Il2ra using anti-IL-2 Receptor alpha/Il2ra antibody (A00214-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: mouse ANA-1 whole cell lysates, Lane 2: mouse NIH/3T3 whole cell lysates, Lane 3: mouse RAW264.7 whole cell lysates, Lane 4: mouse HEPA1-6 whole cell lysates, Lane 5: rat C6 whole cell lysates, Lane 6: rat NRK whole cell lysates, Lane 7: rat RH35 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-IL-2 Receptor alpha/Il2ra antigen affinity purified polyclonal antibody (Catalog # A00214-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for IL-2 Receptor alpha/Il2ra at approximately 50 kDa. The expected band size for IL-2 Receptor alpha/Il2ra is at 50 kDa.
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