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ProductsBack/FITC Anti-Mouse CD279/PD-1 Antibody [29F.1A12]

FITC Anti-Mouse CD279/PD-1 Antibody [29F.1A12]

AGEL1627
Assay Genie
ApplicationsFlow Cytometry
Product group Antibodies
ReactivityMouse
TargetPdcd1
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Overview

  • Supplier
    Assay Genie
  • Product Name
    FITC Anti-Mouse CD279/PD-1 Antibody [29F.1A12]
  • Delivery Days Customer
    9
  • Applications
    Flow Cytometry
  • Applications Supplier
    FCM
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    29F.1A12
  • Conjugate
    FITC
  • Gene ID18566
  • Target name
    Pdcd1
  • Target description
    programmed cell death 1
  • Target synonyms
    Ly101, PD-1, Pdc1, programmed cell death protein 1, mPD-1, programmed cell death 1 protein, protein PD-1
  • Host
    Rat
  • Protein IDQ02242
  • Protein Name
    Programmed cell death protein 1
  • Scientific Description
    FITC Anti-Mouse CD279/PD-1 Antibody [29F.1A12] is a high-performance monoclonal that specifically targets Mouse CD279/PD-1, delivering exceptional specificity and reliability for your research applications. Rigorously validated for FCM, this antibody ensures consistent, reproducible results across multiple experimental platforms. Demonstrates excellent reactivity with Mouse samples, providing researchers with confidence in cross-species compatibility. Conveniently packaged in 100 Tests format to meet your experimental needs. For optimal performance, store at 4°C and maintains stability for 12 months. Backed by rigorous quality control testing to ensure superior performance in your critical research applications.
  • Shelf life instruction
    12 months
  • Source
    Rat
  • Reactivity
    Mouse
  • Reactivity Supplier
    Mouse
  • Storage Instruction
    2°C to 8°C
  • UNSPSC
    41116161

FAQ

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Figure 1. Western blot analysis of PD1/Pdcd1 using anti-PD1/Pdcd1 antibody (A00178-3). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: mouse EL-4 whole cell lysates, Lane 2: mouse spleen tissue lysates, Lane 3: mouse thymus tissue lysates, Lane 4: mouse RAW264.7 whole cell lysates, Lane 5: mouse Ana-1 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PD1/Pdcd1 antigen affinity purified polyclonal antibody (Catalog # A00178-3) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PD1/Pdcd1 at approximately 55 kDa. The expected band size for PD1/Pdcd1 is at 32 kDa.
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