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WB analysis of various sample lysates using GTX54286 MPG antibody. The signal was developed with ECL plus-Enhanced. Dilution : 1:1000 Loading : 25microg per lane
WB analysis of various sample lysates using GTX54286 MPG antibody. The signal was developed with ECL plus-Enhanced. Dilution : 1:1000 Loading : 25microg per lane
WB analysis of various sample lysates using GTX54286 MPG antibody. The signal was developed with ECL plus-Enhanced. Dilution : 1:1000 Loading : 25microg per lane

MPG antibody

GTX54286
GeneTex
TargetMPG
Product group Antibodies
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Overview

  • Supplier
    GeneTex
  • Product Name
    MPG antibody
  • Delivery Days Customer
    7
  • Application Supplier Note
    WB: 1:500 - 1:2000. ICC/IF: 1:50 - 1:100. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID4350
  • Target name
    MPG
  • Target description
    N-methylpurine DNA glycosylase
  • Target synonyms
    AAG, ADPG, APNG, CRA36.1, MDG, Mid1, PIG11, PIG16, anpg, DNA-3-methyladenine glycosylase, 3' end of the Mid1 gene, localized 68 kb upstream the humanzeta globin gene on 16p, 3-alkyladenine DNA glycosylase, 3-methyladenine DNA glycosidase, CRA36.1 (3-methyl-adenine DNA glycosylase), N-methylpurine-DNA glycosylase, MPG, proliferation-inducing protein 11, proliferation-inducing protein 16
  • Host
    Rabbit
  • Isotype
    IgG
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203

Datasheet

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Figure 1. Western blot analysis of AAG/MPG using anti-AAG/MPG antibody (A00037). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HepG2 whole cell lysates, Lane 2: human Jurkat whole cell lysates, Lane 3: human 293T whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-AAG/MPG antigen affinity purified polyclonal antibody (Catalog # A00037) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for AAG/MPG at approximately 37 kDa. The expected band size for AAG/MPG is at 33 kDa.
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