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MPHOSPH9 Antibody (aa539-588)

LS-C291344
LifeSpan BioSciences
ApplicationsWestern Blot, ELISA
Product group Antibodies
TargetMPHOSPH9
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Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    MPHOSPH9 Antibody (aa539-588)
  • Delivery Days Customer
    23
  • Application Supplier Note
    Western Blot: Predicted MW 78 kDa
  • Applications
    Western Blot, ELISA
  • Applications Supplier
    Peptide-ELISA (1:10000), WB (1:500 - 1:1000) Western Blot: Predicted MW 78 kDa
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    1 mg/ml
  • Conjugate
    Unconjugated
  • Estimated Purity
    ...
  • Gene ID10198
  • Target name
    MPHOSPH9
  • Target description
    M-phase phosphoprotein 9
  • Target synonyms
    M-phase phosphoprotein 9; MPP9; MPP-9
  • Host
    Rabbit
  • Isotype
    IgG
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of MPHOSPH9 using anti-MPHOSPH9 antibody (A11350-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Jurkat whole cell lysates, Lane 2: rat brain tissue lysates, Lane 3: mouse brain tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MPHOSPH9 antigen affinity purified polyclonal antibody (Catalog # A11350-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for MPHOSPH9 at approximately 130-140 kDa. The expected band size for MPHOSPH9 is at 130-140 kDa.
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