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Western Blot analysis of A549 cells using MPP9 Polyclonal Antibody
Western Blot analysis of A549 cells using MPP9 Polyclonal Antibody
Western Blot analysis of A549 cells using MPP9 Polyclonal Antibody

MPHOSPH9 Antibody

CSB-PA003268
Cusabio
ApplicationsWestern Blot, ELISA
Product group Antibodies
ReactivityHuman
TargetMPHOSPH9
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Overview

  • Supplier
    Cusabio
  • Product Name
    MPHOSPH9 Antibody
  • Delivery Days Customer
    20
  • Applications
    Western Blot, ELISA
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID10198
  • Target name
    MPHOSPH9
  • Target description
    M-phase phosphoprotein 9
  • Target synonyms
    M-phase phosphoprotein 9; MPP9; MPP-9
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ99550
  • Protein Name
    M-phase phosphoprotein 9
  • Reactivity
    Human
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of MPHOSPH9 using anti-MPHOSPH9 antibody (A11350-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Jurkat whole cell lysates, Lane 2: rat brain tissue lysates, Lane 3: mouse brain tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MPHOSPH9 antigen affinity purified polyclonal antibody (Catalog # A11350-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for MPHOSPH9 at approximately 130-140 kDa. The expected band size for MPHOSPH9 is at 130-140 kDa.
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