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MPP1 Rabbit pAb

A6298
ABclonal
ApplicationsWestern Blot, ELISA
Product group Antibodies
ReactivityHuman, Mouse
TargetMPP1
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Overview

  • Supplier
    ABclonal
  • Product Name
    MPP1 Rabbit pAb
  • Delivery Days Customer
    9
  • Applications
    Western Blot, ELISA
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID4354
  • Target name
    MPP1
  • Target description
    MAGUK p55 scaffold protein 1
  • Target synonyms
    AAG12, DXS552E, EMP55, MRG1, PEMP, 55 kDa erythrocyte membrane protein, aging-associated gene 12, erythrocyte membrane protein p55, membrane palmitoylated protein 1, membrane protein, palmitoylated 1, 55kDa, migration-related gene 1, p55, palmitoylated erythrocyte membrane protein
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ00013
  • Protein Name
    55 kDa erythrocyte membrane protein
  • Reactivity
    Human, Mouse
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of MPP1 using anti-MPP1 antibody (PB10078). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: rat lung tissue lysates, Lane 2: mouse spleen tissue lysates, Lane 3: MCF-7 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MPP1 antigen affinity purified polyclonal antibody (Catalog # PB10078) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for MPP1 at approximately 55 kDa. The expected band size for MPP1 is at 52 kDa.
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