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ProductsBack/MRP2 (B-11): m-IgGkappa BP-HRP Bundle

MRP2 (B-11): m-IgGkappa BP-HRP Bundle

SC-536531
Santa Cruz Biotechnology
ApplicationsImmunoFluorescence, ImmunoPrecipitation, Western Blot, ELISA
Product group Antibodies
ReactivityHuman
TargetABCC2
1 kit
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Overview

  • Supplier
    Santa Cruz Biotechnology
  • Product Name
    MRP2 (B-11): m-IgGkappa BP-HRP Bundle
  • Delivery Days Customer
    5
  • Applications
    ImmunoFluorescence, ImmunoPrecipitation, Western Blot, ELISA
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Gene ID1244
  • Target name
    ABCC2
  • Target description
    ATP binding cassette subfamily C member 2
  • Target synonyms
    ABC30, CMOAT, DJS, MRP2, cMRP, ATP-binding cassette sub-family C member 2, ATP-binding cassette, sub-family C (CFTR/MRP), member 2, canalicular multidrug resistance protein, canalicular multispecific organic anion transporter 1, multidrug resistance-associated protein 2
  • Host
    Mouse
  • Isotype
    IgG2b
  • Reactivity
    Human
  • Storage Instruction
    2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of MRP2/ABCC2 using anti-MRP2/ABCC2 antibody (A00974-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HepG2 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MRP2/ABCC2 antigen affinity purified polyclonal antibody (Catalog # A00974-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for MRP2/ABCC2 at approximately 170-250 kDa. The expected band size for MRP2/ABCC2 is at 174 kDa.
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