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WB analysis of HepG2 lysate (40ug) using MRPS25 antibody at the indicated dilutions.
WB analysis of HepG2 lysate (40ug) using MRPS25 antibody at the indicated dilutions.
WB analysis of HepG2 lysate (40ug) using MRPS25 antibody at the indicated dilutions.

MRPS25 antibody [AT38E7]

GTX53743
GeneTex
ApplicationsWestern Blot, ELISA
Product group Antibodies
TargetMRPS25
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Overview

  • Supplier
    GeneTex
  • Product Name
    MRPS25 antibody [AT38E7]
  • Delivery Days Customer
    9
  • Application Supplier Note
    The antibody has been tested by ELISA, Western blot analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results. Recommended dilution range for Western blot analysis is 1:500 ~ 1:5000. Recommended starting dilution is 1:1000.
  • Applications
    Western Blot, ELISA
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    AT38E7
  • Concentration
    1 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID64432
  • Target name
    MRPS25
  • Target description
    mitochondrial ribosomal protein S25
  • Target synonyms
    28S ribosomal protein S25, mitochondrial; COXPD50; mitochondrial 28S ribosomal protein S25; mitochondrial small ribosomal subunit protein mS25; MRP-S25; RPMS25; S25mt
  • Host
    Mouse
  • Isotype
    IgG2a
  • Protein IDP82663
  • Protein Name
    28S ribosomal protein S25, mitochondrial
  • Scientific Description
    Mammalian mitochondrial ribosomal proteins are encoded by nuclear genes and help in protein synthesis within the mitochondrion. Mitochondrial ribosomes (mitoribosomes) consist of a small 28S subunit and a large 39S subunit. They have an estimated 75% protein to rRNA composition compared to prokaryotic ribosomes, where this ratio is reversed. Another difference between mammalian mitoribosomes and prokaryotic ribosomes is that the latter contain a 5S rRNA. Among different species, the proteins comprising the mitoribosome differ greatly in sequence, and sometimes in biochemical properties, which prevents easy recognition by sequence homology. This gene encodes a 28S subunit protein. A pseudogene corresponding to this gene is found on chromosome 4. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Feb 2016]
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of MRPS25 using anti-MRPS25 antibody (A14080-3). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HEK293 whole cell lysates, Lane 2: human MCF-7 whole cell lysates, Lane 3: human Hela whole cell lysates, Lane 4: human T-47D whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MRPS25 antigen affinity purified polyclonal antibody (Catalog # A14080-3) at 0.25 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for MRPS25 at approximately 20 kDa. The expected band size for MRPS25 is at 20 kDa.
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