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Western Blot analysis of 293 cells using MRP-S25 Polyclonal Antibody
Western Blot analysis of 293 cells using MRP-S25 Polyclonal Antibody
Western Blot analysis of 293 cells using MRP-S25 Polyclonal Antibody

MRPS25 Antibody

CSB-PA003309
Cusabio
ApplicationsWestern Blot, ELISA
Product group Antibodies
TargetMRPS25
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Overview

  • Supplier
    Cusabio
  • Product Name
    MRPS25 Antibody
  • Delivery Days Customer
    20
  • Applications
    Western Blot, ELISA
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID64432
  • Target name
    MRPS25
  • Target description
    mitochondrial ribosomal protein S25
  • Target synonyms
    28S ribosomal protein S25, mitochondrial; COXPD50; mitochondrial 28S ribosomal protein S25; mitochondrial small ribosomal subunit protein mS25; MRP-S25; RPMS25; S25mt
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP82663
  • Protein Name
    28S ribosomal protein S25, mitochondrial
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of MRPS25 using anti-MRPS25 antibody (A14080-3). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HEK293 whole cell lysates, Lane 2: human MCF-7 whole cell lysates, Lane 3: human Hela whole cell lysates, Lane 4: human T-47D whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MRPS25 antigen affinity purified polyclonal antibody (Catalog # A14080-3) at 0.25 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for MRPS25 at approximately 20 kDa. The expected band size for MRPS25 is at 20 kDa.
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