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ProductsBack/MSL2L1 (MSL2) Mouse Monoclonal Antibody [Clone ID: OTI3G5]
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY MSL2 (RC210829, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-MSL2 (1:2000). Positive lysates LY413286 (100ug) and LC413286 (20ug) can be purchased separately from OriGene.
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY MSL2 (RC210829, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-MSL2 (1:2000). Positive lysates LY413286 (100ug) and LC413286 (20ug) can be purchased separately from OriGene.
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY MSL2 (RC210829, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-MSL2 (1:2000). Positive lysates LY413286 (100ug) and LC413286 (20ug) can be purchased separately from OriGene.

MSL2L1 (MSL2) Mouse Monoclonal Antibody [Clone ID: OTI3G5]

TA811223
OriGene
ApplicationsWestern Blot
Product group Antibodies
TargetMSL2
100 ul
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Overview

  • Supplier
    OriGene
  • Product Name
    MSL2L1 (MSL2) Mouse Monoclonal Antibody [Clone ID: OTI3G5]
  • Delivery Days Customer
    14
  • Applications
    Western Blot
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    OTI3G5
  • Gene ID55167
  • Target name
    MSL2
  • Target description
    MSL complex subunit 2
  • Target synonyms
    KBHS, MSL-2, MSL2L1, RNF184, E3 ubiquitin-protein ligase MSL2, E3 ubiquitin-protein transferase MSL2, MSL2-like 1, male-specific lethal 2 homolog, male-specific lethal 2-like 1, male-specific lethal-2 homolog 1, ring finger protein 184
  • Host
    Mouse
  • Isotype
    IgG1
  • Protein IDQ9HCI7
  • Protein Name
    E3 ubiquitin-protein ligase MSL2
  • Scientific Description
    MSL2 mouse monoclonal antibody,clone OTI3G5
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

MSDS

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Figure 1. Western blot analysis of MSL2 using anti-MSL2 antibody (A02712-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HL-60 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MSL2 antigen affinity purified polyclonal antibody (Catalog # A02712-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for MSL2 at approximately 63 kDa. The expected band size for MSL2 is at 63 kDa.
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