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Western blot analysis of extracts of various cell lines, using MSL2 antibody (TA378699) at 1:3000 dilution. - Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. - Lysates/proteins: 25ug per lane. - Blocking buffer: 3% nonfat dry milk in TBST. - Detection: ECL Basic Kit . - Exposure time: 10s.
Western blot analysis of extracts of various cell lines, using MSL2 antibody (TA378699) at 1:3000 dilution. - Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. - Lysates/proteins: 25ug per lane. - Blocking buffer: 3% nonfat dry milk in TBST. - Detection: ECL Basic Kit . - Exposure time: 10s.
Western blot analysis of extracts of various cell lines, using MSL2 antibody (TA378699) at 1:3000 dilution. - Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. - Lysates/proteins: 25ug per lane. - Blocking buffer: 3% nonfat dry milk in TBST. - Detection: ECL Basic Kit . - Exposure time: 10s.

MSL2L1 (MSL2) Rabbit Polyclonal Antibody

TA378699
OriGene
ApplicationsWestern Blot
Product group Antibodies
TargetMSL2
100 ul
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Overview

  • Supplier
    OriGene
  • Product Name
    MSL2L1 (MSL2) Rabbit Polyclonal Antibody
  • Delivery Days Customer
    14
  • Applications
    Western Blot
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Gene ID55167
  • Target name
    MSL2
  • Target description
    MSL complex subunit 2
  • Target synonyms
    E3 ubiquitin-protein ligase MSL2; E3 ubiquitin-protein transferase MSL2; male-specific lethal 2 homolog; male-specific lethal 2-like 1; male-specific lethal-2 homolog 1; MSL-2; MSL2L1; MSL2-like 1; ring finger protein 184; RNF184
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ9HCI7
  • Protein Name
    E3 ubiquitin-protein ligase MSL2
  • Scientific Description
    MSL2 Rabbit polyclonal Antibody
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

MSDS

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Figure 1. Western blot analysis of MSL2 using anti-MSL2 antibody (A02712-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HL-60 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MSL2 antigen affinity purified polyclonal antibody (Catalog # A02712-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for MSL2 at approximately 63 kDa. The expected band size for MSL2 is at 63 kDa.
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Western Blot Positive WB detected in: U251 whole cell lysate All lanes: MSL2 antibody at 5microg/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 63, 55 kDa Observed band size: 63 kDa
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Western blot analysis in human cell line MOLT-4.
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