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ProductsBack/MSN Polyclonal Antibody
Western blot analysis of extracts of various cell lines using Moesin Polyclonal Antibody at 1:1000 dilution.
Western blot analysis of extracts of various cell lines using Moesin Polyclonal Antibody at 1:1000 dilution.
Western blot analysis of extracts of various cell lines using Moesin Polyclonal Antibody at 1:1000 dilution.

MSN Polyclonal Antibody

E-AB-60663
Elabscience
Product group Antibodies

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Overview

  • Supplier
    Elabscience
  • Product Name
    MSN Polyclonal Antibody
  • Delivery Days Customer
    12
  • Certification
    Research Use Only
  • Scientific Description
    Moesin (for membrane-organizing extension spike protein) is a member of the ERM family which includes ezrin and radixin. ERM proteins appear to function as cross-linkers between plasma membranes and actin-based cytoskeletons. Moesin is localized to filopodia and other membranous protrusions that are important for cell-cell recognition and signaling and for cell movement.
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of MSN using anti-MSN antibody (A00766-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human MDA-MB-453 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MSN antigen affinity purified polyclonal antibody (Catalog # A00766-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for MSN at approximately 78KD. The expected band size for MSN is at 66KD.
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