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ProductsBack/MSR1 antibody [2F8] (Biotin)
FACS analysis of mouse peritoneal macrophages using GTX43968 MSR1 antibody [2F8] (Biotin).
FACS analysis of mouse peritoneal macrophages using GTX43968 MSR1 antibody [2F8] (Biotin).
FACS analysis of mouse peritoneal macrophages using GTX43968 MSR1 antibody [2F8] (Biotin).

MSR1 antibody [2F8] (Biotin)

GTX43968
GeneTex
ApplicationsFlow Cytometry
Product group Antibodies
TargetMsr1
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Overview

  • Supplier
    GeneTex
  • Product Name
    MSR1 antibody [2F8] (Biotin)
  • Delivery Days Customer
    9
  • Application Supplier Note
    FACS: Neat. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    Flow Cytometry
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    2F8
  • Concentration
    0.1 mg/ml
  • Conjugate
    Biotin
  • Gene ID20288
  • Target name
    Msr1
  • Target description
    macrophage scavenger receptor 1
  • Target synonyms
    macrophage acetylated LDL receptor I and II; macrophage scavenger receptor types I and II; MR; MRS-A; MS; MSR; MSR-A; S; Sc; Scara1; scavenger receptor type A; Scvr; SR-A; SR-AI; SR-AII
  • Host
    Rat
  • Isotype
    IgG2b
  • Protein IDP30204
  • Protein Name
    Macrophage scavenger receptor types I and II
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203

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Lane 1: Mouse Spleen tissue lysates; Lane 2: Mouse Lung tissue lysates; Lane 3: Mouse Liver tissue lysates; Lane 4: Mouse Placenta tissue lysates ; Lane 5: Mouse Cerebrum tissue lysates; Lane 6: Mouse Raw264.7 cell lysates; Lane 7: Mouse NIH/3T3 cell lysates; Lane 8: Rat Liver tissue lysates; Lane 9: Rat Cerebrum tissue lysates probed with MSR1 Polyclonal Antibody, Unconjugated (bs-24362R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
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Figure 1. Western blot analysis of CD204/Msr1 using anti-CD204/Msr1 antibody (A02349-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: mouse RAW264.7 whole cell lysates, Lane 2: mouse ANA-1 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CD204/Msr1 antigen affinity purified polyclonal antibody (Catalog # A02349-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CD204/Msr1 at approximately 75-90 kDa. The expected band size for CD204/Msr1 is at 71 kDa.
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