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MST1 Antibody (YA2368, Rabbit)

HY-P82623
MedChem Express
TargetMST1
Product group Antibodies
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Overview

  • Supplier
    MedChem Express
  • Product Name
    MST1 Antibody (YA2368, Rabbit)
  • Delivery Days Customer
    5
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Gene ID4485
  • Target name
    MST1
  • Target description
    macrophage stimulating 1
  • Target synonyms
    D3F15S2, DNF15S2, HGFL, MSP, NF15S2, hepatocyte growth factor-like protein, hepatocyte growth factor-like protein homolog, macrophage stimulating 1 (hepatocyte growth factor-like), macrophage-stimulating protein
  • Host
    Rabbit
  • Isotype
    IgG
  • Scientific Description
    MST1 Antibody (YA2368) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to MST1.
  • Storage Instruction
    -20°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of MST1 using anti-MST1 antibody (A01069-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human Hela cell lysate, Lane 2: human MCF-7 cell lysate, Lane 3: human HepG2 cell lysate, Lane 4: human SK-OV-3 cell lysate, Lane 5: human A549 cell lysate, Lane 6: rat kidney tissue lysate, Lane 7: mouse kidney tissue lysate. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MST1 antigen affinity purified polyclonal antibody (Catalog # A01069-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for MST1 at approximately 80KD. The expected band size for MST1 is at 80KD.
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IHC image of CSB-PA015055LA01HU diluted at 1:400 and staining in paraffin-embedded human gastric cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
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