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ProductsBack/MSTN / GDF8 / Myostatin Antibody

MSTN / GDF8 / Myostatin Antibody

LS-C812473
LifeSpan BioSciences
ApplicationsWestern Blot, ELISA
Product group Antibodies
TargetMSTN
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Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    MSTN / GDF8 / Myostatin Antibody
  • Delivery Days Customer
    23
  • Application Supplier Note
    Applications should be user optimized.
  • Applications
    Western Blot, ELISA
  • Applications Supplier
    ELISA (1:10000), WB (1:500 - 1:2000) Applications should be user optimized.
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Conjugate
    Unconjugated
  • Estimated Purity
    ...
  • Gene ID2660
  • Target name
    MSTN
  • Target description
    myostatin
  • Target synonyms
    GDF8, MSLHP, growth/differentiation factor 8, myostatin-b
  • Host
    Mouse
  • Isotype
    IgG1
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of GDF8/Myostatin/MSTN using anti-GDF8/Myostatin/MSTN antibody (A00926-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human CACO-2 whole cell lysates, Lane 2: rat skeletal muscle tissue lysates, Lane 3: mouse skeletal muscle tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-GDF8/Myostatin/MSTN antigen affinity purified polyclonal antibody (Catalog # A00926-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for GDF8/Myostatin/MSTN at approximately 50KD. The expected band size for GDF8/Myostatin/MSTN is at 50KD.
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