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MT-CO2 Polyclonal Antibody

MBS8566798
MyBioSource
Product group Antibodies
Price on request
0.1 ml
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Overview

  • Supplier
    MyBioSource
  • Product Name
    MT-CO2 Polyclonal Antibody
  • Delivery Days Customer
    16
  • Certification
    Research Use Only
  • Estimated Purity
    Affinity Purification
  • Isotype
    IgG
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of MT-CO2 using anti-MT-CO2 antibody (A03631-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human K562 whole cell lysates, Lane 2: human MCF-7 whole cell lysates, Lane 3: human Hela whole cell lysates, Lane 4: human Jurkat whole cell lysates, Lane 5: human A431 whole cell lysates, Lane 6: human Hacat whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MT-CO2 antigen affinity purified polyclonal antibody (Catalog # A03631-1) at 0.25 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for MT-CO2 at approximately 21 kDa. The expected band size for MT-CO2 is at 21 kDa.
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Immunohistochemical staining of human cerebellum, kidney, liver and stomach using Anti-MT-CO2 antibody HPA051505 (A) shows similar protein distribution across tissues to independent antibody HPA054758 (B).
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Various whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with MTCO2 antibody [GT1293] (GTX03205) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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