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Western Blot analysis of HT29 cells using ND5 Polyclonal Antibody
Western Blot analysis of HT29 cells using ND5 Polyclonal Antibody
Western Blot analysis of HT29 cells using ND5 Polyclonal Antibody

MT-ND5 Antibody

CSB-PA003382
Cusabio
ApplicationsWestern Blot, ELISA
Product group Antibodies
ReactivityHuman
TargetND5
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Overview

  • Supplier
    Cusabio
  • Product Name
    MT-ND5 Antibody
  • Delivery Days Customer
    20
  • Applications
    Western Blot, ELISA
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID4540
  • Target name
    ND5
  • Target description
    NADH dehydrogenase subunit 5
  • Target synonyms
    MTND5, NADH dehydrogenase subunit 5
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP03915
  • Protein Name
    NADH-ubiquinone oxidoreductase chain 5
  • Reactivity
    Human
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of MT-ND5 using anti-MT-ND5 antibody (A03488-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human HepG2 whole cell lysates, Lane 3: human Raji whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MT-ND5 antigen affinity purified polyclonal antibody (Catalog # A03488-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for MT-ND5 at approximately 67 kDa. The expected band size for MT-ND5 is at 67 kDa.
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