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MT-ND5 Antibody (Internal)

LS-C368770
LifeSpan BioSciences
ApplicationsImmunoPrecipitation, Western Blot
Product group Antibodies
ReactivityBovine, Canine, Human, Mouse, Porcine, Rabbit, Rat, Sheep, Zebra Fish
TargetND5
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Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    MT-ND5 Antibody (Internal)
  • Delivery Days Customer
    23
  • Applications
    ImmunoPrecipitation, Western Blot
  • Applications Supplier
    IP (1:10 - 1:100), WB (1:500 - 1:1000)
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    1 mg/ml
  • Conjugate
    Unconjugated
  • Estimated Purity
    ...
  • Gene ID4540
  • Target name
    ND5
  • Target description
    NADH dehydrogenase subunit 5
  • Target synonyms
    MTND5, NADH dehydrogenase subunit 5
  • Host
    Rabbit
  • Reactivity
    Bovine, Canine, Human, Mouse, Porcine, Rabbit, Rat, Sheep, Zebra Fish
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

FAQ

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Figure 1. Western blot analysis of MT-ND5 using anti-MT-ND5 antibody (A03488-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human HepG2 whole cell lysates, Lane 3: human Raji whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MT-ND5 antigen affinity purified polyclonal antibody (Catalog # A03488-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for MT-ND5 at approximately 67 kDa. The expected band size for MT-ND5 is at 67 kDa.
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