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MTA2 Polyclonal Antibody

RD219422A
Reddot Biotech
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman, Mouse
TargetMTA2
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Overview

  • Supplier
    Reddot Biotech
  • Product Name
    MTA2 Polyclonal Antibody
  • Delivery Days Customer
    5
  • Applications
    Western Blot, ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    0.7 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID9219
  • Target name
    MTA2
  • Target description
    metastasis associated 1 family member 2
  • Target synonyms
    MTA1L1, PID, metastasis-associated protein MTA2, MTA1-L1 protein, metastasis -associated gene 1-like 1, metastasis associated gene family, member 2, metastasis-associated 1-like 1, metastasis-associated protein 2, p53 target protein in deacetylase complex
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDO94776
  • Protein Name
    Metastasis-associated protein MTA2
  • Scientific Description
    Metastasis-associated protein MTA2,also named as MTA1L1 or PID,is a 668 amino acid protein,which contains one BAH domain,one ELM2 domain,one GATA-type zinc finger and one SANT domain. MTA2 localizes in the nucleus and is widely expressed in many tissues. MTA2 may be involved in the regulation of gene expression as repressor and activator. - This is a MTA2 Polyclonal Antibody from Reddot Biotech. This product is for Research Use Only.
  • Reactivity
    Human, Mouse
  • Storage Instruction
    -20°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of MTA2 using anti-MTA2 antibody (A03073-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human HEK293 whole cell lysates, Lane 3: human K562 whole cell lysates, Lane 4: rat liver tissue lysates, Lane 5: mouse heart tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MTA2 antigen affinity purified polyclonal antibody (Catalog # A03073-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for MTA2 at approximately 75KD. The expected band size for MTA2 is at 75KD.
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