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ProductsBack/MTMR2 Mouse Monoclonal Antibody [Clone ID: OTI1F10]
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY MTMR2 (RC208703, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-MTMR2. Positive lysates LY414151 (100ug) and LC414151 (20ug) can be purchased separately from OriGene.
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY MTMR2 (RC208703, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-MTMR2. Positive lysates LY414151 (100ug) and LC414151 (20ug) can be purchased separately from OriGene.
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY MTMR2 (RC208703, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-MTMR2. Positive lysates LY414151 (100ug) and LC414151 (20ug) can be purchased separately from OriGene.

MTMR2 Mouse Monoclonal Antibody [Clone ID: OTI1F10]

CF502200
OriGene
ApplicationsWestern Blot
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetMTMR2
Price on request
100 ug
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Overview

  • Supplier
    OriGene
  • Product Name
    MTMR2 Mouse Monoclonal Antibody [Clone ID: OTI1F10]
  • Delivery Days Customer
    14
  • Applications
    Western Blot
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    OTI1F10
  • Gene ID8898
  • Target name
    MTMR2
  • Target description
    myotubularin related protein 2
  • Target synonyms
    CMT4B, CMT4B1, phosphatidylinositol-3,5-bisphosphate 3-phosphatase MTMR2, phosphatidylinositol-3,5-bisphosphate 3-phosphatase, phosphatidylinositol-3-phosphatase, phosphatidylinositol-3-phosphate phosphatase, phosphoinositide-3-phosphatase
  • Host
    Mouse
  • Isotype
    IgG1
  • Protein IDQ13614
  • Protein Name
    Phosphatidylinositol-3,5-bisphosphate 3-phosphatase MTMR2
  • Scientific Description
    Carrier-free (BSA/glycerol-free) MTMR2 mouse monoclonal antibody, clone OTI1F10 (formerly 1F10)
  • Reactivity
    Human, Mouse, Rat
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

MSDS

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Figure 1. Western blot analysis of MTMR2 using anti-MTMR2 antibody (A04202-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human Caco-2 whole cell lysates, Lane 3: human HL-60 whole cell lysates, Lane 4: rat testis tissue lysates, Lane 5: mouse testis tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MTMR2 antigen affinity purified polyclonal antibody (Catalog # A04202-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for MTMR2 at approximately 73 kDa. The expected band size for MTMR2 is at 73 kDa.
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