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ProductsBack/mtTFA antibody [HL2012]
mtTFA antibody [HL2012] detects mtTFA protein at mitochondria by immunohistochemical analysis. Sample: Paraffin-embedded human colon cancer. mtTFA stained by mtTFA antibody [HL2012] (GTX637913) diluted at 1:100. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
mtTFA antibody [HL2012] detects mtTFA protein at mitochondria by immunohistochemical analysis. Sample: Paraffin-embedded human colon cancer. mtTFA stained by mtTFA antibody [HL2012] (GTX637913) diluted at 1:100. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
mtTFA antibody [HL2012] detects mtTFA protein at mitochondria by immunohistochemical analysis. Sample: Paraffin-embedded human colon cancer. mtTFA stained by mtTFA antibody [HL2012] (GTX637913) diluted at 1:100. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

mtTFA antibody [HL2012]

GTX637913
GeneTex
ApplicationsWestern Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityHuman
TargetTFAM
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Overview

  • Supplier
    GeneTex
  • Product Name
    mtTFA antibody [HL2012]
  • Delivery Days Customer
    9
  • Application Supplier Note
    WB: 1:500-1:3000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    Western Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    HL2012
  • Concentration
    1 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID7019
  • Target name
    TFAM
  • Target description
    transcription factor A, mitochondrial
  • Target synonyms
    MTDPS15, MTTF1, MTTFA, TCF6, TCF6L1, TCF6L2, TCF6L3, transcription factor A, mitochondrial, mitochondrial transcription factor 1, mitochondrial transcription factor A, transcription factor 6, transcription factor 6-like 1, transcription factor 6-like 2 (mitochondrial transcription factor), transcription factor 6-like 3
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ00059
  • Protein Name
    Transcription factor A, mitochondrial
  • Scientific Description
    This gene encodes a key mitochondrial transcription factor containing two high mobility group motifs. The encoded protein also functions in mitochondrial DNA replication and repair. Sequence polymorphisms in this gene are associated with Alzheimers and Parkinsons diseases. There are pseudogenes for this gene on chromosomes 6, 7, and 11. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Aug 2012]
  • Reactivity
    Human
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of mtTFA using anti-mtTFA antibody (PB9447). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: Caco-2 whole cell lysates, Lane 2: SW620 whole cell lysates, Lane 3: PANC-1 whole cell lysates, Lane 4: rat brain tissue lysates, Lane 5: mouse brain tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-mtTFA antigen affinity purified polyclonal antibody (Catalog # PB9447) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for mtTFA at approximately 24KD. The expected band size for mtTFA is at 24KD.
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