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WB analysis of membrane enriched extracts (30 microg lysate) of Neuro-2a (Lane 1), U-87 MG (Lane 2), IMR-32 (Lane 3), SK-N-AS (Lane 4), U-2 OS (Lane 5) and tissue extracts of Mouse Cerebellum (Lane 6) and Rat Brain (Lane 7) using GTX17934 Mu Opioid receptor antibody. Dilution : 1:250
WB analysis of membrane enriched extracts (30 microg lysate) of Neuro-2a (Lane 1), U-87 MG (Lane 2), IMR-32 (Lane 3), SK-N-AS (Lane 4), U-2 OS (Lane 5) and tissue extracts of Mouse Cerebellum (Lane 6) and Rat Brain (Lane 7) using GTX17934 Mu Opioid receptor antibody. Dilution : 1:250
WB analysis of membrane enriched extracts (30 microg lysate) of Neuro-2a (Lane 1), U-87 MG (Lane 2), IMR-32 (Lane 3), SK-N-AS (Lane 4), U-2 OS (Lane 5) and tissue extracts of Mouse Cerebellum (Lane 6) and Rat Brain (Lane 7) using GTX17934 Mu Opioid receptor antibody. Dilution : 1:250

Mu Opioid receptor antibody

GTX17934
GeneTex
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
Product group Antibodies
TargetOPRM1
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Overview

  • Supplier
    GeneTex
  • Product Name
    Mu Opioid receptor antibody
  • Delivery Days Customer
    9
  • Application Supplier Note
    WB: 1:250. ICC/IF: 1:250. FACS: 1:20. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    Flow Cytometry, ImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID4988
  • Target name
    OPRM1
  • Target description
    opioid receptor mu 1
  • Target synonyms
    LMOR; MOP; MOR; MOR1; M-OR-1; mu opiate receptor; mu opioid receptor hMOR-1a; mu-type opioid receptor; OPRM
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP35372
  • Protein Name
    Mu-type opioid receptor
  • Scientific Description
    This gene encodes one of at least three opioid receptors in humans; the mu opioid receptor (MOR). The MOR is the principal target of endogenous opioid peptides and opioid analgesic agents such as beta-endorphin and enkephalins. The MOR also has an important role in dependence to other drugs of abuse, such as nicotine, cocaine, and alcohol via its modulation of the dopamine system. The NM_001008503.2:c.118A>G allele has been associated with opioid and alcohol addiction and variations in pain sensitivity but evidence for it having a causal role is conflicting. Multiple transcript variants encoding different isoforms have been found for this gene. Though the canonical MOR belongs to the superfamily of 7-transmembrane-spanning G-protein-coupled receptors some isoforms of this gene have only 6 transmembrane domains. [provided by RefSeq, Oct 2013]
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of OPRM1 using anti-OPRM1 antibody (A00737-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human SH-SY5Y whole cell lysates, Lane 2: human K562 whole cell lysates, Lane 3: human Jurkat whole cell lysates, Lane 4: human U251 whole cell lysates, Lane 5: rat brain tissue lysates, Lane 6: rat heart tissue lysates, Lane 7: mouse brain tissue lysates, Lane 8: mouse heart tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-OPRM1 antigen affinity purified polyclonal antibody (Catalog # A00737-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for OPRM1 at approximately 70 kDa. The expected band size for OPRM1 is at 45 kDa.
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