Bio-Connect

Anti-Muscarinic Acetylcholine Receptor 2/CHRM2 Antibody

ORB107617
Biorbyt
ApplicationsWestern Blot, ImmunoHistoChemistry
Product group Antibodies
TargetCHRM2
Sign in to order and to see your custom pricing.
Large volume orders?
Order with a bulk request

Overview

  • Supplier
    Biorbyt
  • Product Name
    Muscarinic Acetylcholine Receptor 2/CHRM2 Antibody
  • Delivery Days Customer
    16
  • Application Supplier Note
    Immunohistochemistry (Paraffin-embedded Section), 0.5-1microg/ml, Human, Rat, Mouse Western blot, 0.1-0.5microg/ml, Human, Mouse, Rat. Add 0.2ml of distilled water will yield a concentration of 500ug/ml
  • Applications
    Western Blot, ImmunoHistoChemistry
  • Applications Supplier
    IHC, WB
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    500 ug/ml
  • Conjugate
    Unconjugated
  • Gene ID1129
  • Target name
    CHRM2
  • Target description
    cholinergic receptor muscarinic 2
  • Target synonyms
    7TM receptor; acetylcholine receptor, muscarinic 2; HM2; muscarinic acetylcholine receptor M2; muscarinic M2 receptor
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP08172
  • Protein Name
    Muscarinic acetylcholine receptor M2
  • Scientific Description
    Anti-Muscarinic Acetylcholine Receptor 2/CHRM2 Antibody
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    12352203

Related products

Product group Antibodies
Everest Biotech
ApplicationsWestern Blot, ELISA
TargetCHRM2
  • SizePrice
Product group Antibodies
Assay Genie
TargetCHRM2
  • SizePrice
Product group Antibodies
GeneTex
ApplicationsImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
TargetCHRM2
  • SizePrice
Product group Antibodies
MedChem Express
TargetCHRM2
  • SizePrice
Immunofluorescence staining of SH-SY5Y cells with CSB-PA005382PA01HU at 1:50, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
Product group Antibodies
Cusabio
CHRM2 AntibodyCSB-PA005382PA01HU
ApplicationsImmunoFluorescence, ELISA
TargetCHRM2
  • SizePrice
Mouse brain lysates probed with ChRM2 Polyclonal Antibody, Unconjugated (bs-0236R) at 1:500 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:10000 for 60 min at 37˚C.
Product group Antibodies
Bioss Antibodies
ApplicationsImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
TargetCHRM2
  • SizePrice
Anti-CHRM2 Antibody
Product group Antibodies
Antibodies.com
ApplicationsImmunoFluorescence, Western Blot, ELISA
  • SizePrice
Product group Antibodies
RayBiotech
ApplicationsImmunoFluorescence, Western Blot
TargetCHRM2
  • SizePrice
Product group Antibodies
LifeSpan BioSciences
ApplicationsImmunoFluorescence, Western Blot, ImmunoHistoChemistry
TargetCHRM2
  • SizePrice
Figure 1. Western blot analysis of CHRM2 using anti-CHRM2 antibody (A02733). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. lane 1: rat brain tissue lysates, lane 2: mouse brain tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CHRM2 antigen affinity purified polyclonal antibody (Catalog # A02733) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CHRM2 at approximately 52KD. The expected band size for CHRM2 is at 52KD.
Product group Antibodies
Boster Bio
ApplicationsWestern Blot
TargetCHRM2
  • SizePrice